A rat pup model of cerebral palsy induced by prenatal inflammation and hypoxia  

作　　者：Yanrong Hu Gang Chen Hong Wan Zhiyou Zhang Hong Zhi Wei Liu Xinwei Qian Mingzhao Chen Linbao Wen Feng Gao Jianxin Li Lihui Zhao 

机构地区：[1]Postdoctoral Research Station,School of Basic Medicine, CAMA and PUMC [2]Postdoctoral Research Station, the People's Hospital of Xinjiang Uygur Autonomous Region [3]Department of Neurosurgery,the Fourth People's Hospital of Wuxi (The Fourth Affiliated Hospital of Soochow University) [4]Beijing Neurosurgical Institute [5]Department of Neurosurgery, Xinjiang Autonomous Region People's Hospital [6]Department of Neurosurgery,Xinjiang Autonomous Region People's Hospital [7]Department of Neurology,Xinjiang Autonomous Region People's Hospital [8]Department of Pathology,Xinjiang Autonomous Region People's Hospital

出　　处：《Neural Regeneration Research》2013年第9期817-824,共8页中国神经再生研究（英文版）

基　　金：funded by the National Natural Science Foundation of China,No.30960393;the Key Foundation in Science and Technology of Xinjiang Uygur Autonomous Region,No.200633128(2);the Youth Science and Technology Foundation of Health Department of Xinjiang Uygur Autonomous Region,No.2007Y26;the Science and Technology Foundation of Health Bureau of Wuxi,No.ML201211

摘　　要：Animal models of cerebral palsy established by simple infection or the hypoxia/ischemia method cannot effectively simulate the brain injury of a premature infant. Healthy 17-day-pregnant Wistar rats were intraperitoneally injected with lipopolysaccharide then subjected to hypoxia. The pups were used for this study at 4 weeks of age. Simultaneously, a hypoxia/ischemia group and a control group were used for comparison. The results of the footprint test, the balance beam test, the water maze test, neuroelectrophysiological examination and neuropathological examination demonstrated that, at 4 weeks after birth, footprint repeat space became larger between the forelimbs and hindlimbs of the rats, the latency period on the balance beam and in the Morris water maze was longer, place navigation and ability were poorer, and the stimulus intensity that induced the maximal wave amplitude of the compound muscle action potential was greater in the lipopolysaccharide/hypoxia and hypoxia/ischemia groups than in the control group. We observed irregular cells around the periventricular area, periventricular leukomalacia and breakage of the nuclear membrane in the lipopolysacchadde/hypexia and hypoxia/ischemia groups. These results indicate that we successfully established a Wistar rat pup model of cerebral palsy by intraperitoneal injection of lipopolysaccharide and hypoxia.Animal models of cerebral palsy established by simple infection or the hypoxia/ischemia method cannot effectively simulate the brain injury of a premature infant. Healthy 17-day-pregnant Wistar rats were intraperitoneally injected with lipopolysaccharide then subjected to hypoxia. The pups were used for this study at 4 weeks of age. Simultaneously, a hypoxia/ischemia group and a control group were used for comparison. The results of the footprint test, the balance beam test, the water maze test, neuroelectrophysiological examination and neuropathological examination demonstrated that, at 4 weeks after birth, footprint repeat space became larger between the forelimbs and hindlimbs of the rats, the latency period on the balance beam and in the Morris water maze was longer, place navigation and ability were poorer, and the stimulus intensity that induced the maximal wave amplitude of the compound muscle action potential was greater in the lipopolysaccharide/hypoxia and hypoxia/ischemia groups than in the control group. We observed irregular cells around the periventricular area, periventricular leukomalacia and breakage of the nuclear membrane in the lipopolysacchadde/hypexia and hypoxia/ischemia groups. These results indicate that we successfully established a Wistar rat pup model of cerebral palsy by intraperitoneal injection of lipopolysaccharide and hypoxia.

关 键 词：neural regeneration brain injury HYPOXIA lipopolysaccharide animal models cerebral palsy watermaze test neuroelectrophysiology histopathology grants-supported paper photographs-containing paper neuroregeneration 

分 类 号：R742.3[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]