肝细胞癌中GPC3和Notch1表达的关系  

Relationship between glypican-3 and Notch1 expressions in hepatocellular carcinoma

作　　者：彭璠莉 吴自勍[1,2] 李学农[1,2] 

机构地区：[1]南方医科大学南方医院病理科//广东省分子肿瘤病理学重点实验室,广东广州510515 [2]南方医科大学基础医学院病理系//广东省分子肿瘤病理学重点实验室,广东广州510515

出　　处：《南方医科大学学报》2013年第4期590-592,597,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81071659)~~

摘　　要：目的通过观察肝细胞癌组织中磷脂酰肌醇蛋白聚糖3(GPC3)与Notch1的表达情况,探讨两者在肝细胞癌中表达的相互关系。方法应用免疫组化方法、计算机图像分析等技术检测30例肝细胞癌组织中GPC3和Notch1的表达情况,并用SPSS软件系统进行统计学分析。结果肝细胞癌组织分化程度越高,GPC3表达越低;分化程度越低,GPC3表达越高,差异有显著性(P<0.05)或高度显著性(P<0.01)。肝细胞癌组织分化程度越高,Notch1表达越高;分化程度越低,Notch1表达越低,差异有显著性(P<0.05)或高度显著性(P<0.01)。GPC3与Notch1的表达呈高度显著性负相关(rp=-0.607,P=0.000;r=-0.692,P=0.000)。结论在肝细胞癌中,GPC3与Notch1的表达呈现负相关性,提示两者间可能存在相互调节的机制,共同影响肝细胞癌的发生发展。Objective To investigate the correlation between the expressions of glypican-3（GPC3） and Notch1 and their roles in the tumorigenesis and progression of hepatocellular carcinoma（HCC）.Methods Immunohistochemistry and computerized image analysis were utilized to quantitatively detect the expressions of GPC3 and Notch1 in 30 HCC tissue specimens.Results In the 30 HCC specimens,GPC3 expression decreased significantly as the grade of tumor differentiation increased（P0.05 or P0.01）,while Notch1 expression presented with a reverse pattern of changes（P0.05 or P0.01）.An obvious negative correlation was found between the expressions of GPC3 and Notch1 in HCC tissues（rp=-0.607,P=0.000;r=-0.692,P=0.000）. Conclusion The expressions of GPC3 and Notch1 show a negative correlation in HCC,suggesting a possible mechanism for mutual regulation between them to contribute to the tumorigenesis and progression of HCC.

关 键 词：癌 肝细胞 磷脂酰肌醇蛋白聚糖3 NOTCH1 免疫组化 

分 类 号：R735.7[医药卫生—肿瘤]