培养基成分对鸡腿菇液态培养产漆酶的影响  

Effect of Different Component of Medium on Laccase Production by Coprinus Comatus

作　　者：娄钧翼[1] 李芬[1] 潘益芳[1] 赵成龙[1] 金燕南[1] 章琴燕[1] 顾小敏[1] 林海萍[1] 

机构地区：[1]亚热带森林培育国家重点实验室培育基地生物农药高效制备技术浙江省工程实验室浙江农林大学,浙江临安311300

出　　处：《中国食品学报》2013年第3期135-139,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省大学生科技创新活动计划(新苗人才计划)项目(2012R412042)

摘　　要：为提高漆酶产量,进一步拓展漆酶开发利用空间,本文研究碳源、氮源、诱导剂、铜离子4种成分对鸡腿菇液态培养漆酶产量的影响。以ABTS为底物,用紫外分光光度法测定漆酶活性,连续测定20d左右。测定结果表明:供试碳源中,蔗糖促进鸡腿菇产漆酶效果最明显,在产酶液态基础培养基中加入2%蔗糖时,鸡腿菇漆酶产量最高达1827.5U/L;氮源中酵母粉效果最明显,在加有2%蔗糖的产酶液态培养基中加入0.2%酵母粉时,漆酶最高产量为679.1667U/L;愈创木酚在供试诱导剂中促进效果最明显,在加有2%蔗糖、0.2%酵母粉的产酶基础培养基中加入0.1mmol/L愈创木酚诱导剂时,鸡腿菇产漆酶最高达1479.792U/L;在加有2%蔗糖,0.2%酵母粉的产酶基础培养基中加入3.0mmol/L硫酸铜时,漆酶产量达到最大,为1154.167U/L。Coprinus comatus was inoculated in different liquid medium with different medium component including carbon sources, nitrogen sources, inducers or/and different concentration of Cu^2＋ so as to investigate the effect of the above four medium components on laccase production by Coprinus comatus. The laccase activity was detected in the UV- spectrophotometric method used ABTS as the substrate and lasted for 20 days or so. The results showed that sucrose, yeast extract, Guaiacol and 3.0 mmol/L Cu^2＋ had distinct effect on laccase production by Coprinus comatus among their counterparts. In the medium with 2% sucrose the laccase activity peak reached 1 827.5 U/L. While added 0.2% yeast extract in the medium with 2% sucrose, the laccase activity peak was 679.1667 U/L. The highest laccase activity with 1 479.792 U/L was detected in the medium of 2% sucrose, 0.2% yeast extract and 0.1 mmol/L Guaiacol. When 0.2, 0.4, 0.6, 0.8, 1.0, 3.0, 5.0 mmol/L CuSO4 was added to the medium with 2% sucrose, 0.2% yeast extract, it was found that the laccace activity reached its peak of 1 154.167 U/L in 3.0 mmol/L Cu^2＋ medium. Based on the results, it is expected to contribute to further study of optimizing the production of laccase by Coprinus comatus.

关 键 词：鸡腿菇 漆酶 培养基成分 液态培养 

分 类 号：TQ925[轻工技术与工程—发酵工程]