万寿菊花中叶黄素提取工艺的优化  

Optimization of Extraction Technology of Lutein from Marigold Flower

作　　者：李凤伟[1] 刘丽娜[1] 

机构地区：[1]盐城工学院化学与生物工程学院,江苏盐城224051

出　　处：《湖北农业科学》2012年第18期4094-4096,4108,共4页Hubei Agricultural Sciences

摘　　要：为了研究采用ASE-100加速溶剂萃取仪提取万寿菊(Tagetes erecta L.)花中叶黄素的最佳工艺条件,采用五因素五水平正交试验提取叶黄素,用高效液相色谱法测定皂化后的叶黄素产量。用ASE-100加速溶剂萃取仪提取叶黄素的最佳试验参数为提取温度80℃、料液比1∶30(m/V,g∶mL)、静态萃取时间10 min、静态周期数3、冲洗体积70%。To optimize the technical conditions for extracting lutein from marigold flower by ASE-100 Accelerated Solvent Extractor,five factors-five levels orthogonal design was applied.And the yeild of lutein was determined by HPLC after saponification.The optimal lutein extraction conditions from marigold flower were extraction temperature,80 ℃;solid to liquid ratio,1∶30（m/V,g∶mL）;static extraction time,10 min;static cycle,3;flush volume,70%.

关 键 词：万寿菊(Tagetes ERECTA L )花 叶黄素 提取 正交试验 

分 类 号：Q949[生物学—植物学]