考马斯亮蓝染色法测定大豆茎叶中蛋白质含量  

Determination of Protein in Soybean Stems and Leaves by Bradford Method

作　　者：杨正坤[1] 王秀丽[1] 龙施华[1] 郝再彬[1] 单展展[1] 周菲菲[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541006

出　　处：《湖北农业科学》2012年第20期4610-4612,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金资助项目(31060193)

摘　　要：应用考马斯亮蓝染色法测定大豆茎叶中蛋白质含量。结果表明,该法测定大豆茎叶中蛋白质含量是可行的,其RSD为0.25%～1.27%,回收率为94.9%～106.4%,大豆茎叶蛋白质平均含量为7.673%和11.315%。该方法简便快速,灵敏度高、重现性好、准确率相对较高,可用于微量可溶性蛋白质含量的测定。The content of protein in soybean leaves and stems was determined by Bradford method.The result showed that it was feasible determining protein content in soybean leaves and stems by this method,as the RSD was 0.25%～1.27%;the recovery reached 94.9%～106.4%.The average protein content of soybean leaves and stems were 7.673% and 11.315%,respectively.This method was simple,rapid,reproducible,sensitive and accurate,thus was an effective method for determining trace soluble protein.

关 键 词：大豆茎叶 蛋白质含量 考马斯亮蓝染色法 

分 类 号：TS63[轻工技术与工程]