二氧化硫诱导酵母细胞死亡的信号途径研究  

Investigation of the Signaling Pathways Involved in Sulfar Doxide Induced Cell Death in Yeast

作　　者：乔瑞芳[1] 仪慧兰[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2012年第4期722-725,共4页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(30870454;30470318);高等学校博士学科点专项科研基金(20070108007);山西省留学回国基金(2009022)

摘　　要：以酵母细胞为材料,研究SO2衍生物(Na2SO3-NaHSO3混合液,3∶1,mmol.L-1∶mmol.L-1)致细胞死亡的信号途径.结果表明,在浓度25～100mmol.L-1范围内,SO2衍生物处理可引起酵母细胞死亡;一定浓度的抗氧化剂抗坏血酸(AsA)和Ca2+螯合剂乙二醇双四乙酸(EGTA)与SO2衍生物共同作用时,酵母细胞死亡率显著降低.研究结果表明,一定浓度的SO2可诱导酵母细胞死亡,胁迫可能通过诱导胞内活性氧和Ca2+升高,引发细胞死亡.The effects of SO2 derivatives on cells death were investigated in model eukaryote yeast.The yeast cells were treated with various chemicals,stained with methylene blue dye,and then examined with a microscope.The randomly selected views on the tested yeast cells were monitored to determine the number of dead cells and the total number of scored cells.SO2 derivatives,a mixture of sodium sulfite and sodium bisulfite（3∶1,mmol·L-1∶mol·L-1）,significantly induced cell death at concentrations from 25 to 100 mmol·L-1.However,the percentage of cell death decreased when yeast cells exposed to SO2 derivatives combined with either ascorbic acid（AsA,1 mmol·L-1） or EGTA（Ca2＋ chelator,0.5 mmol·L-1）.The results of the present study indicated that SO2 derivatives caused cell death via reactive oxygen species（ROS） production,as well as intracellular Ca2＋ level increase in yeast cells,both of them could mediate cell death.

关 键 词：酵母细胞 SO2衍生物 细胞死亡 ROS CA2+ 

分 类 号：Q932[生物学—微生物学]