酶试剂法快速检测常用农药残留的比较研究  

Comparative Study on Enzyme Reagent Method for Rapid Detection of Common Pesticide Residues

作　　者：易涛[1] 赵明明[2] 路磊[2] 丁华[2] 周有祥[2] 李书谦[2] 

机构地区：[1]华中农业大学食品科学技术学院,武汉430070 [2]湖北省农业科学院农业质量标准与检测技术研究所,武汉430064

出　　处：《湖北农业科学》2012年第21期4873-4875,共3页Hubei Agricultural Sciences

基　　金：国家农业科技成果转化资金项目(2011GB2D10002);湖北省农业科技创新中心资助项目(2011-620-000-001)

摘　　要：根据果蔬中农药残留检出的实际情况,采用市场上的3个商品化农残酶速测试剂盒对常见农药(水胺硫磷、敌敌畏、呋喃丹、毒死蜱、三唑磷、灭多威、氯氟氰菊酯、甲氰菊酯、百菌清、腐霉利和三唑酮)进行了灵敏度试验。结果表明,酶试剂对腐霉利、氯氟氰菊酯、甲氰菊酯、百菌清、三唑酮5种农药无抑制反应或灵敏度较差,而对其余6种农药的反应较为灵敏,其灵敏度依次为敌敌畏、呋喃丹、三唑磷、毒死蜱、水胺硫磷和灭多威。The common residue of pesticides in fruits and vegetables,including isocarbophos,dichlorvos,carbofuran,chlorpyrifos,triazophos,methomyl,cyhalothrin,fenpropathrin,chlorothalonil,procymidone and triazolone,were analyzed by the 3 commercial enzyme inhibition kits with the pesticide residue rapid analyzer.The results showed that any enzyme reagent inhibition kit was not sensitive to procymidone,cyhalothrin,fenpropathrin,chlorothalonil and triazolone,it was only sensitive to six pesticides,the sensitivity to the six pesticides in order was dichlorvos,carbofuran,triazophos,chlorpyrifos,isocarbophos and methomyl.

关 键 词：酶抑制法 快速检测 农药 灵敏度 

分 类 号：O657.32[理学—分析化学] Q556.1[理学—化学]