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作 者:Aly E1 Sheikha Didier Montet
机构地区:[1]Department of Food Science and Technology, Minufiya University, Shibin El Kom 32511, Egypt [2]Halal Products Research Institute (HPRI), Putra University of Malaysia (UPM), Serdang 43400, Selangor Darul Ehsan, Malaysia [3]UMR Qualisud, Center of International Cooperation in Agronomic Research for Development (CIRAD), Montpellier Cedex 534398, France
出 处:《Journal of Life Sciences》2012年第12期1334-1342,共9页生命科学(英文版)
摘 要:Abstract: One of the great concerns of the customers is the traceability of the products. The authors proposed to link microbial ecology to geographical origin of foodstuffs by a molecular technique joined to an image analysis. Molecular techniques employing 16S and 28S rDNA profiles generated by PCR-DGGE were used to detect the variation in microbial community (bacteria, fungi) of Pangasius fish from Viet Nam harvested in different aquaculture farms and during different seasons and Shea tree fruits from five different districts in Mali. The bacterial DNA profiles from Pangasius fish and the fungal DNA profile from Shea tree fruits were specific to each place of production and could be used as a biological bar code certifying the origin of fish and fruit. To follow the product during processing, the authors proposed to identify and validate some pertinent biological markers which come from the environment of the food to assure their traceability during international trade. It is new analytical method which permits to determine the origin of food or to follow them during international trade.
关 键 词:Geographical origin FOODSTUFF PCR-DGGE microbial community DNA profile biological bar-code.
分 类 号:TS201.6[轻工技术与工程—食品科学] X172[轻工技术与工程—食品科学与工程]
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