搅拌桨类型对彩绒革盖菌产草酸脱羧酶的影响  

Effects of mixing propellers on production of oxalate decarboxylase by Trametes versicolor

作　　者：宫搏阳[1] 曹张军[1] 洪枫[1,2] 杨雪霞[1] 

机构地区：[1]东华大学化学化工与生物工程学院微生物工程与工业生物技术研究组 [2]东华大学生态纺织教育部重点实验室,上海201620

出　　处：《工业微生物》2013年第2期13-16,共4页Industrial Microbiology

基　　金：国家自然科学基金(30671650);生态纺织教育部重点实验室开放课题(Eco-op-2009-09);中央高校基本科研业务费专项资金资助项目

摘　　要：研究了在相同发酵工艺的条件下,发酵罐中5种搅拌桨对彩绒革盖菌发酵生产草酸脱羧酶的影响。实验结果表明,桨的类型对产酶有显著影响,四斜叶搅拌桨剪切力最小,发酵生产草酸脱羧酶的体积酶活、蛋白比酶活和干重比酶活分别为77.9 U/L、9.5 U/mg和22.6 U/g,比普遍使用的六平叶圆盘涡轮搅拌桨获得的酶活分别提高了406%、533%和70%。The effects of 5 different mixing propellers on the production of oxalate decarboxylase （OxDC） of a filamentous fungus Trametes versicolor were investigated. The results showed that the types of mixing propellers had remarkable influ- ences on the production of OxDC. A propeller with four inclined blades generated the minimum cutting force. The volume activity of OxDC reached 77.9 U/L, and the specific activity reached 9.5 U/rag protein and 22.6 U/g dry biomass, which was improved by 406% , 533% and 70% respectively compared with those of a turbine propeller with six straight blades.

关 键 词：彩绒革盖菌 草酸脱羧酶 发酵 搅拌桨 剪切力 

分 类 号：TQ925[轻工技术与工程—发酵工程]