超高效液相色谱-串联质谱法测定植物提取物中的4种黄曲霉毒素  

Determination of four aflatoxins in plant extract by ultra-high performance liquid chromatography-tandem mass spectrometry

作　　者：孔祥虹[1,2] 高军刚[2] 何强 何芳[2] 

机构地区：[1]陕西出入境检验检疫局,西安710068 [2]陕西师范大学食品工程与营养科学学院,西安710061

出　　处：《食品科技》2013年第4期325-329,共5页Food Science and Technology

基　　金：国家质检公益性科研项目(201010040);陕西省科技计划项目(2008k01-06);农业部科技项目(CARS-28苹果产业技术体系)

摘　　要：建立了超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定植物提取物中的4种黄曲霉毒素(AFT)的方法。样品用乙腈提取、免疫亲和柱净化,采用UPLC-MS/MS进行分析。在0.1～5.0μg/L的范围内线性关系良好,相关系数r>0.994;B1、B2、G1和G2检出限(LOD)分别为0.05、0.03、0.03μg/kg和0.08μg/kg,回收率在65.8%～98.2%之间,相对标准偏差(RSD)在7.6%～18.3%之间。结果表明,该方法灵敏度高、选择性强且稳定性好,可用于植物提取物中黄曲霉毒素的检测。An easy method for the determination of aflatoxins B1,B2,G1 and G2 in plant extract by ultra performance liquid chromatography coupled with tandem mass spectrometry（UPLC-MS/MS） has been developed.Aflatoxins were extracted with acetonitrile and then it was purified by immunoaffinity column,and the extracts were determined by UPLC-MS/MS.The good correlation coefficients（r0.994） of the four aflatoxins were obtained within their respective linear ranges（0.1～5.0 μg/L）.The determination limits for 4 kinds of aflatoxins were 0.05,0.03,0.03,0.08 μg/kg,the average recoveries were between 65.8%～98.2%,the relative standard deviations（RSDs） were in the range from 7.6% to18.3%.The results indicate that the developed method is sensitive,fast,accurate,and can be applied for the determination of four aflatoxins in plant extract.

关 键 词：超高效液相色谱-串联质谱 植物提取物 黄曲霉毒素 

分 类 号：TS207.5[轻工技术与工程—食品科学]