辣椒叶多酚抗氧化及抗炎活性研究  

Study on anti-oxidation and anti-inflammatory activity of Capsicum annuum leaves polyphenols

作　　者：张友仁[1,2] 唐涛[2] 黄昀[2] 李勇[2,3] 谢乐[2,4] 詹欣[1,2] 唐正海[1,2] 唐劲天[2] 

机构地区：[1]北京中医大学中药学院,北京100102 [2]清华大学工程物理学粒子技术与辐射成像教育部重点实验室,北京100084 [3]兰州交通大学化学与生物工程学院,甘肃兰州730070 [4]湖南中医药大学附属湖南省脑科医院神经内科,湖南长沙410208

出　　处：《食品工业科技》2013年第8期346-349,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金青年基金(81102889)

摘　　要：通过ABTS+自由基、DPPH自由基清除能力、铁还原能力、小鼠耳肿胀实验考查辣椒叶多酚提取物粗提物及纯化物的体外抗氧化及抗急性炎症活性。结果表明,辣椒叶多酚提取物具有一定的抗氧化能力,其纯化物清除ABTS+自由基、DPPH自由基和铁还原能力的IC50分别为0.700、0.643、0.758mg/mL,与对照品VC浓度处于同一数量级,说明辣椒叶多酚提取物是一种有效的天然抗氧化剂;600mg/kg剂量的辣椒叶多酚的粗提物及纯化物,均对二甲苯所致小鼠耳肿胀有极显著抑制作用,抑制率分别为59.84%、62.69%(p<0.01);炎症组织PGE2含量从32.41A/g可分别降至19.27、16.51A/g(p<0.05);实验小鼠血清肝损伤指标ALT(谷丙转氨酶)和AST(谷草转氨酶)的监测表明辣椒叶多酚提取物具有较高安全性。实验结果表明辣椒叶多酚提取物具有一定的抗氧化及抗急性炎症活性且安全性较高,为进一步开发利用辣椒资源提供了理论依据。Polyphenol extracted both crude and refined had anti-oxidant and anti-acute inflammatory activity by the experiments of ABTS^＋ radical and DPPH radical scavenging,iron reduction and mice＇s auricle swelling- inhibition. The refined extract＇s median inhibitory concentration（ICso） of ABTS^＋ radical scavenging,DPPH radical scavenging and iron reduction ability were 0.700,0.643,0.758mg/mL,respectively. Results were in the same order of magnitude of the reference substance Vc which indicated that polyphenols extract could be used as an effective natural anti-oxidants. The extract,both crude and refined with the dose of 600mg/kg,could significantly inhibit auricle swelling induced by xylene in mouse and the swelling inhibition ratios were 59.84% and 62.69% （p〈0.01）,respectively. Furthermore,the content of PGE2 in mice edema ear was respectively decreased from 32.41A/g to 19.27A/g, 16.51A/g（p〈0.05）. The extract were also proved to be non-toxic for the assay of serum biomarkers （including alanine transaminase（ALT） and aspartate transanimase （AST） for liver injury） during the 5d. In summary,this study indicated that polyphenols extract of Capsicum annuum leaves had high anti-oxidant and anti-acute inflammatory activity but non-toxic,which provided a theoretical basis for the further research of the comprehensive development of Capsicum annuum resources.

关 键 词：辣椒叶 多酚 抗氧化 抗炎 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]