食品中6种新型农药的超高效液相色谱-串联质谱法测定  

Determination of Six New Pesticides in Foods by UPLC-MS/MS

作　　者：陈意光 罗海英 罗东辉 冼燕萍 吴玉銮 

机构地区：[1]广州市质量监督检测研究院,国家加工食品质量监督检验中心(广州),广州市食品安全检测技术重点实验室,广州市食品安全风险动态监测与预警研究中心,广东广州510110

出　　处：《江南大学学报（自然科学版）》2013年第2期227-233,共7页Joural of Jiangnan University (Natural Science Edition)　

基　　金：国家质量监督检验检疫总局科技计划项目(2009QK327);广东省科技计划项目(2007B023001001);广州市科信局科技计划项目(穗科信字[2011]233-34号)

摘　　要：建立了蕃茄、苹果汁、凉茶、米粉、蜂蜜和牛奶中氟虫酰胺、氯虫酰胺、烯啶虫胺、吡虫啉、虫酰肼和抑食肼6种新型农药的UPLC-MS/MS测定方法。蕃茄、凉茶样品用乙腈和乙酸乙酯混合溶剂提取;蜂蜜和米粉样品先用水溶解后再用混合溶剂提取;牛奶样品用乙腈提取;苹果汁样品高速离心后取上层清液。待测液在正离子模式下用电喷雾电离-串联质谱仪测定,方法检出限范围为0.01～2μg/kg。在20μg/kg和160μg/kg 2个质量分数水平进行加标回收实验,平均回收率在68.4%～129%之间。方法准确、高效,适合该6种食品基质中6种农药残留的测定。An UPLC-MS/MS method was developed for simultaneous determination of flubendiamide, chlorantraniliprole, nitenpyram, imidaeloprid, tebufenozide, RH5849 in foods. Tomato and herbal tea samples were extracted with mixed solvent of aeetonitrile and ethyl acetate （ V： V = 4 ： 1 ）. Honey samples and rice flour samples were dissolved by water and then extracted with mixed solvent of acetonitrile and ethyl acetate. Milk samples were extracted by acetonitrile. Apple juice samples were centrifuged then tested. Reverse phase UPLC-tandem mass operated under positive ion mode was employed to analyze the compounds. The detection limits were from 0.01 μg/kg to 2 μg/kg. The average recoveries were from 68.4% to 129% at the spiked levels of 20 μg/kg and 160μg/kg. It was an accurate and effective method to determinate the six new pesticides in foods.

关 键 词：食品 新型农药 超高效液相色谱-串联质谱 

分 类 号：TS207.5[轻工技术与工程—食品科学]