α-亚麻酸对转化生长因子β1诱导的肝星状细胞的影响  

Effect of ALA treatment on cell proliferation, secretion of types Ⅰ and Ⅲ collagen, and α-SMA expression in rat hepatic satellite cells treated with transforming growth factor β1

作　　者：王莉娟[1] 吴金明[1] 方红龙[1] 刘扬[1] 黄智铭[1] 

机构地区：[1]温州医学院附属第一医院消化内科,浙江省温州市325000

出　　处：《世界华人消化杂志》2013年第9期804-808,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨α-亚麻酸(α-linolenic acid,ALA)对转化生长因子1(transforming growth factor-β,TGF-β1)诱导的肝星状细胞(hepatic satellite cell,HSC)增殖及分泌功能的影响.方法:采用活化的HSC作为研究模型,将传代的细胞分为5组,以MTT比色法观察ALA对HSC的增殖效应,以ELASA检测细胞培养上清液中Ⅰ型及Ⅲ型胶原的含量,以Westernblot法检测α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的表达.结果:TGF-β1能诱导HSC增殖并促进胶原的分泌,ALA可不同程度抑制TGF-β1所诱导的HSC增殖及Ⅰ、Ⅲ型胶原和α-SMA的表达(P<0.01),且呈剂量依赖性(P<0.05).结论:ALA在高浓度时对TGF-β1能诱导的HSC起抑制作用,可能对脂肪性肝纤维化及其引起的肝硬化有治疗作用.AIM： To investigate the effect of treatment with α-linolenic acid （ALA） on cell proliferation, secre-tion of types Ⅰ and Ⅲ collagen, and α-smooth muscle actin （α-SMA） expression in transforming growth factor beta 1 （TGF-β1）-treated rat hepatic satellite cells （HSCs）.METHODS： Cultured HSCs were divided into five groups： control group, TGF-β1 group （5 ng/ mL） and three TGF-β1 plus ALA groups （treated with TGF-β1 plus 50, 150 or 250 ng/mL of ALA）. Cell proliferation was assessed by MTT assay. The levels of types Ⅰ and Ⅲ collagen were measured by ELISA. Western blot was used to detectthe expression of α-smooth muscle actin. RESULTS： TGF-β1 induced cell proliferation and increased the secretion of collagen in HSCs. Compared to the TGF-β1 group, treatment with different doses of ALA inhibited cell proliferation and decreased the secretion of types Ⅰ and Ⅲ collagen and expression of α-SMA （all P 0.05）.CONCLUSION： Treatment with ALA inhibits TGF-β1-induced cell proliferation, secretion of types Ⅰ and Ⅲ collagen, and α-SMA expression in rat HSCs.

关 键 词：Α-亚麻酸 转化生长因子Β1 肝星状细胞 脂肪性肝纤维化 肝硬化 

分 类 号：R575.2[医药卫生—消化系统]