一种纳豆芽孢杆菌的分子鉴定及纳豆激酶基因克隆  

Molecular Indentification of Bacillus Subtilis Natto and Clone Gene of Nattokinase

作　　者：李建强[1,2] 石洪凌[2] 肖冬光[1] 

机构地区：[1]天津科技大学生物工程学院,天津300000 [2]唐山师范学院生命科学系,河北唐山063000

出　　处：《唐山师范学院学报》2013年第2期37-40,共4页Journal of Tangshan Normal University

摘　　要：以实验室保藏的一株具有溶解血栓能力的芽孢杆菌(LJQ-NK)为出发菌株,通过形态学分析和16SrRNA比对,对菌株进行鉴定,更进一步利用聚合酶链式反应(PCR)克隆纳豆激酶DNA片段⑤16SrRNA比对。结果表明LJQ-NK与已报道的纳豆激酶16SrRNA序列有99.9%以上的同源性,初步认定为一株纳豆芽孢杆菌。克隆获得1 120 bp的DNA片段,应用DNAMAN软件分析,与已报道的纳豆激酶编码基因(GI29164926)存在两个碱基的差异,编码纳豆激酶开放阅读框区域序列完全相同。LJQ-NK, a strain with ability of fibrinolysis was identified by molecular biology method. Sequence analysis shows that the homology of 16S rRNA between LJQ-NK and Bacillus subtilis natto was exceeded 99. 9%. The putative nattokinase cDNA was cloned from LJQ-NK using RT-PCR and a 1 126 bp cDNA clone was obtained. Result of BLAST-X sequence homology search show that two bases of putative nattokinase cDNA clone were differed from nattokinase and the ORF sequence ofnattokinase between LJQ-NK and Bacillus subtilis natto were identical.

关 键 词：纳豆芽孢杆菌 纳豆激酶 16S rRNA比对 

分 类 号：Q785[生物学—分子生物学]