肿瘤转移抑制基因KISS-1和NM23-H1的原核表达与纯化  

Prokaryotic Expression and Purification of Metastasis Suppressor Gene KISS-1 and NM23-H1

作　　者：左芳雷[1] 冯秀娟[1] 陈尚武[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《生物技术通报》2013年第3期186-191,共6页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目(31071507)

摘　　要：从人胎盘组织和乳腺癌细胞中克隆得到肿瘤转移抑制基因KISS-1和NM23-H1,经Blast比对分析与GenBank公布序列同源性达100%。NM23-H1在大肠杆菌中成功表达,大部分为可溶性蛋白,占总蛋白50%以上,经纯化后纯度达到90%;而KISS-1需要与载体的部分序列融合才能够得到有效表达,表达产物为不可溶的包涵体蛋白,占包涵体总量的70%以上,经纯化后KISS-1蛋白可能形成多聚体结构。Metastasis suppressor gene KISS-1 and NM23-H1 were cloned from human placenta and breast cancer cell, share 100% identity with that published in GenBank. NM23-H1 was successfully expressed in E.coli, the major amount of protein was soluble, accounting for more than 50% of total protein, the purified protein has 90% purity ; however, KISS-1 should be fused to the sequence from expression vector then can effectively expressed, the expressed protein was insoluble inclusion body, accounting for more than 70% of total insoluble protein, after purification, KISS-1 may formed into polymeric structures.

关 键 词：肿瘤转移抑制基因 KISS-1 NM23-H1 表达 纯化 

分 类 号：R73-37[医药卫生—肿瘤]