应用基因组原位杂交及RFLP标记鉴定小麦中的大麦染色体  

Identification of the Barley Chromosome in Wheat by GISH and RFLP

作　　者：原亚萍[1] 陈孝[1] 肖世和[1] 辛志勇[1] 张增艳[1] 林志珊[1] 马有志[1] 胡汉桥[2] 

机构地区：[1]中国农业科学院作物育种栽培研究所 [2]中国人民解放军军需大学,长春130062

出　　处：《Acta Genetica Sinica》2000年第12期1080-1083,T003,共5页

基　　金：国家自然科学基金资助

摘　　要：用生物素（Biotin-16-dUTP)标记的大麦Betzes基因组DNA作探针，以普通小麦中国春总DNA作封阻进行基因组原位杂交（Genome in situ hybridization,简称GISH），从13株小麦-大麦杂交后代中鉴定出2个含有3条大麦Betzes 2H染色体的材料（2n＝43）；2个2H单体异代换系（2n＝42）；7个2H二体异代换系（2n＝42）。用已定位在小麦第2部分同源群短臂上的探针psr131进行RFLP分析，结果表明大麦Betzes、代换系A5有1条区别于小麦中国春的特异带，A5的2条2A染色体被大麦Betzes的2条2H染色体所代换。GISH及RFLP的准确鉴定及小麦－大麦2H二体异代换系的首次获得，为向小麦导入2H染色体上的有用基因提供了宝贵材料。The barley chromosome in wheat was identified by genomic in situ hybridization (GISH) inwhich biotin labelled total genomic DNA of barley Betzes was used as probe and the unlabelled total DNA of common wheat Chinese Spring (CS) as blocking DNA. A series of wheat materials were tested as follows: two disomic alien substitution and monosomic alien addition lines, 2n＝43;two monosomic alien substitution lines, 2n＝42; seven disomic alien substitution lines, 2n＝42. RFLP probe psr131 on the short arm of the homoelogous group 2 was used to analyze the barley chromosome in wheat. The result indicated that there was a same band in barley Betzes and substitution line A5. The chromosome 2A of A5 was substituted by the chromosome 2H of barley. These materials will be useful in transfering the valuable genes in the chromosome 2H to wheat.

关 键 词：基因组原位杂交 RFLP 小麦 大麦 异代换系 

分 类 号：Q943[生物学—植物学]