十二碳二元酸的发酵研究  

Studies on Fermentation of Decane 1,10-dicarboxylic Acid(DC_(12))

作　　者：任刚[1] 陈远童[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《生物工程学报》2000年第2期198-202,共5页Chinese Journal of Biotechnology

基　　金：国家"九五"科技攻关项目!( 96 C0 3 0 3 0 4)

摘　　要：热带假丝酵母(Candidatropicalis)UH248菌株在单因子实验的基础上经过正交实验后,以正十二烷发酵生成十二碳二元酸的产酸量从817g/L提高到1082g/L。而其二元酸产品的纯度(>97%)满足工业生产的需要。吐温60010%、尿素012%、维生素B2150μg/L、青霉素150μ/mL、丙氨酸050%的浓度时也对十二碳二元酸产量有促进作用。用一株以UH248为母株诱变筛选的新的优良突变株HP12菌株,在最佳条件下,20m3罐中发酵151h,十二碳二元酸的平均产量达2021g/L。Candida tropicalis strain UH 2 48 can increase the production of decane 1,10 dicarboxylic acid(DC 12 ) yield by 30% (from 81.7g/l to 108.2g/l) after regulating the fermentation condition.The purity of the DC 12 is above 97%,which can meet the criteria of the succeeding synthesized industrial productions.Emulsifier can not only destroy the structure of the cell membrane in some degree,but also can enhance the permeability of the cell membrane.Furthermore,the structure of the emulsifier itself can also affect its function.Tween 60(0.10%) can enhance the production of DC 12 tremendously.Urea,as the nitrogen resource,can affect the activity of cytochrome P450 enzyme.Some biochemistry and biophysics factors,such as penicillin,vitamin B 2 and alanine,can increase the yield of DC 12 during the process of fermentation.Ferment in 20m 3 fermentator,under the optimal condition,the average yield of DC 12 using the strain HP 12 which derived from UH 2 48, is 202.1g/L.

关 键 词：热带假丝酵母 十二碳二元酸 发酵 

分 类 号：Q815[生物学—生物工程]