灵芝孢子油对免疫低下模型小鼠的免疫调节作用  

Immunoregulatory function of Ganoderma spore oil in immunocompromised mice

作　　者：易有金[1,2,3] 胡瞬[1,4] 熊兴耀[2] 刘东波[2] 钟英丽[2] 易传祝 

机构地区：[1]湖南农业大学食品科学技术学院,长沙410128 [2]湖南农业大学湖南省作物种质创新与资源利用重点实验室,长沙410128 [3]湖南农业大学食品科学技术学院食品科学与生物技术重点实验室,长沙410128 [4]湘南学院预防医学与医学检验系,湖南郴州423000 [5]湖南省疾病预防与控制中心,长沙410005

出　　处：《浙江大学学报（农业与生命科学版）》2013年第2期161-166,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：湖南省人事厅湖南省博士后基金资助项目(0971111)

摘　　要：通过给昆明种小鼠隔天皮下注射环磷酰胺建立免疫功能低下模型,用灵芝孢子油灌胃小鼠30d,分别测定小鼠的免疫器官指数、脾淋巴细胞转化率和迟发型变态反应、抗体生成细胞数和血清溶血素水平、自然杀伤细胞活性,以研究灵芝孢子油对小鼠免疫功能的调节作用.结果表明:灵芝孢子油能显著提高免疫低下模型小鼠的脾脏指数(P<0.05)和胸腺指数(P<0.05);提高小鼠脾淋巴细胞转化率和迟发型变态反应水平(P<0.05);增强血清溶血素水平和抗体生成细胞数(P<0.05);改善小鼠自然杀伤细胞的活性(P<0.05).说明灵芝孢子油能提高免疫低下小鼠的体液免疫、细胞免疫和非特异性免疫功能,对机体特异性及非特异性免疫功能均具有显著调节作用.Ganoderma lucidum as a Chinese traditional medicine has been used as a medicine and food for a very long time. Ganoderma spore oil, a sort of lipin extracted from Ganoderma spore （the reproductive organs of Ganoderma lucidum）, is rich in ganodenic acid, unsaturated fatty acid, Reishi polysaccharides and so forth. The reports on immunoregulation and antioxidation of Ganoderma spore oil are few at present. Cyclophosphamide （CPA） is a common antineoplastic agent which can cause immunodepression. In order to reduce clinically immunocompromised side effects caused by CPA, the immune-deficient mouse model was established to study the immunoregulation effect of Ganoderma spore oil on immunocompromised mice. Ganoderma spore oil was given to hypo-immune model induced by injecting Kunming mice with CPA. The mice were randomly divided into nomal control group, model control group, Ganoderma spore oil group. Ganoderma spore oil group accepted 0.01 mL/g of 150 mg/kg Ganoderrna spore oil for 30 d. Normal control group and model control group were given corn oil of 0.01 mL/g for 30 d. Model control group and Ganoderrna spore oil group were given 40 mg/kg CPA once a day for 1, 3, 5, 17, 19, 21 d respectively, to establish immune- deficient mouse model. Nomal control group was injected subcutaneously with normal saline. The spleen and thymus index, lymphocyte transformation and delayed-type hypersensitivity （DTH）, hemolysin antibody level and hemolysin content, natural killer （NK） cell activity were measured at the 30th day. The results showed that compared with normal control group, the measured parameters were low for model control group. However, the related parameters for Ganoderma spore oil group were higher than model control group and the difference was obvious （P〈0.05）, indicating that the Ganoderma spore oil could increase the spleen index, thymus index, lymphocyte transformation and DTH level, and improve the content of serum hemolysin in immunocompromised mice. In addition, the Ganoderma spore o

关 键 词：灵芝孢子油 环磷酰胺 免疫调节 小鼠 

分 类 号：Q291[生物学—细胞生物学]