星点设计-效应面法优选南瓜多糖提取工艺  

Optimization of Polysaccharide Extraction from Pumpkin by Central Composite Design/Response Surface Methodology

作　　者：陈立江[1] 王永杰[1] 刘宇[1] 高飞[1] 焉喜臣[1] 

机构地区：[1]辽宁大学药学院,辽宁沈阳110036

出　　处：《食品科学》2013年第8期107-112,共6页Food Science

基　　金：辽宁大学"211工程"三期建设项目

摘　　要：目的:采用星点设计-效应面法优选南瓜多糖的提取工艺,并选用适宜的大孔树脂进行纯化,探讨适合工业化生产的南瓜多糖最优的提取纯化工艺。方法:本实验以热水浸提法提取南瓜多糖,采用单因素试验和星点试验设计,研究料液比、提取时间、提取温度、提取次数以及醇沉条件对南瓜多糖浸膏得率的影响,并采用乙醇反复沉淀的方法以及大孔吸附树脂对南瓜多糖进行纯化从而得到进一步纯化的南瓜多糖。结果:南瓜多糖提取最佳工艺为36倍量的水在84℃温度下提取3.2h,提取3次,然后浓缩至1/3体积、以3倍体积95%乙醇醇沉。选用AB-8型大孔吸附树脂进行纯化,纯化的最优条件为20℃条件下上柱,初始液质量浓度取2.84mg/mL,以5BV上柱,5BV的20%乙醇溶液洗脱,最终得到纯化后南瓜多糖的含量可达到60%以上。结论:星点设计-效应面法优选南瓜多糖的提取工艺,方法简便,预测性良好。实验选用AB-8型大孔吸附树脂对南瓜多糖进行纯化。Objective： Central composite design/response surface methodology was employed to optimize the extraction of polysaccharides from pumpkin and a suitable type of macroporous resin was identified for the purification of crude polysaccharides. Methods： A hot water extraction method was proposed for polysaccharide extraction from pumpkin. One-factor-at-a-time method and central composite design/response surface methodology were used to examine the effects of extraction conditions including solid-to-liquid ratio, time, temperature and repeated extraction as well as alcohol precipitation conditions on the extraction efficiency of polysaccharides. Purified polysaccharides were obtained through repeated alcohol precipitation and purification by macroporous resign chromatography. Results： The optimum extraction process of pumpkin polysaccharides was found to be three extraction cycles with a 36-fold volume of hot water at 84 ℃ for 3.2 h each followed by evaporation until one-third of the original volume and precipitation with a three-fold volume of alcohol. AB-8 resin was chosen to purify the crude polysaccharides through adsorption at 20 ℃ with an initial sample concentration of 2.84 mg/mL and a sample loading amount of 5 BV and desorption with 5 BV of 20% alcohol. The purified products contained over 60% polysaccharides. Conclusion： The optimized extraction process is simple and reliable. AB-8 resin is suitable for the purification of crude polysaccharides from pumpkin.

关 键 词：南瓜多糖 提取 星点设计-效应面法 大孔吸附树脂 分离纯化 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]