3种HPLC检测器测定单甘酯含量的方法比较  

Comparison of Three Detectors Used for HPLC Determination of Monoglycerides

作　　者：方敏[1] 赵谋明[1] 赵强忠[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《食品科学》2013年第8期161-163,共3页Food Science

基　　金：国家自然科学基金青年科学基金项目(20806030);粤港关键领域重点突破项目(2009A020700003)

摘　　要：比较高效液相色谱-紫外检测法(HPLC-UV)、高效液相色谱-示差折光检测法(HPLC-RI)、高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定乳浊液中单甘酯的含量。结果表明:UV法检测单甘酯,杂质干扰严重,样品信号不强,灵敏度低;RI法只有在较高的质量浓度范围内才有较好的线性关系,不适合用于检测较低质量浓度的单甘酯;ELSD法检测单甘酯含量,单甘酯质量浓度在0.040～5.000mg/mL范围内有良好的线性关系,R2为0.9998,样品回收率达到92.94%,相对标准偏差为2.24%,变异系数为2.41%。HPLC-ELSD法灵敏,准确,重复性好,可用于乳浊液中单甘酯含量的测定。Comparison of HPLC coupled with each of the detectors ultraviolet detector （UV）, differential refractive index detector （RI） and evaporative light scattering detector （ELSD） was carried out for the determination of monoglycerides. Serious interference from impurities, low signal intensity and weak sensitivity were observed when using UV detection. However, RI detection showed good linearity over a high concentration range despite not being suitable for the detection of low-concentration samples. Similarly, ELSD detection exhibited excellent linearity over the concentration range of 0.040 to 5.000 mg/mL, with a correlation coefficient of 0.9998; the recovery of ELSD detection was 92.94%, with a relative standard deviation of 2.24% and a coefficient of variation of 2.41%. HPLC-ELSD was sensitive, accurate and repeatable and applicable for the determination of monoglycerides in emulsion.

关 键 词：单甘酯 含量测定 高效液相色谱-紫外检测 高效液相色谱-示差折光检测法 高效液相色谱-蒸发光散射检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]