几种云南核桃内种皮黄酮及多酚含量的测定  

Determination of the Content of Several Yunnan Walnut Kernel Pellicle Flavonoids and Polyphenols

作　　者：张春梅[1] 陈朝银[2] 林玉萍[1] 樊启猛[1] 张天财[2] 赵声兰[1] 

机构地区：[1]云南中医学院,云南昆明650500 [2]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《云南中医学院学报》2013年第2期10-13,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家科技支撑计划项目(NO:2011BAD46B03);云南省科技计划项目(NO:2009EB081;2011AB006)

摘　　要：目的测定云南三种核桃内种皮的总黄酮及总多酚含量。方法以核桃内种皮为原料，芦丁为对照品，用比色法测定核桃内种皮的总黄酮含量；以没食子酸为对照品，用Folin—Cioeaileu比色法测定核桃内种皮的多酚含量。结果芦丁在0-0．1mg／mL、没食子酸在0～50μg／mL范围内，吸收值与浓度均呈良好的线性关系。云南麻果核桃、米甸核桃和草果核桃内种皮的黄酮类化合物含量分别为15．48％、17．33％和15．23％，酚类化合物含量分别为57．58％、31．25％和24．01％．结论测定的三种云南核桃内种皮含有丰富的黄酮类化合物和多酚类化合物，且含量随品种的不同而不同，其中麻果核桃内种皮的多酚含量高达57．58％，具有深入研究和开发利用价值。Objective To determine the content of flavonoids and polyphenols in the three walnut kernel pellicle from Yunnan different areas of. Methods With the walnut kernel pellicle as raw material,flavonoids in walnut kernel pelliele by eolorimetric method with rutin as eontrol,polyphenols in the walnut seed capsule was determined by Folin-Ciocaileu eolorimetric method with gallic acid as the standard. Results Rutin in 0-0.1mg/mL,gallic acid in 0-50μg/mL range,absorption value showed a good linear relationship with concentration. The flavonoids in the Yunnan Maguo walnut,Midian walnut and Caoguo walnut kernel pellicle are 15.48%, 17.33% and 15.23%,respectively,the phenolic compounds were 57.58%,31.25% and 24.01%. Conclusion The three kinds of Yunnan walnut kernel pellicle contain abundant flavonoids and polyphenolic eompoundse. And polyphenols in the Maguo walnut kernel pellicle is as high as 57.58%,with the potential of research and development.

关 键 词：核桃内种皮 黄酮类 多酚类 含量 测定 

分 类 号：R284.1[医药卫生—中药学]