Niche-less maintenance of HSCs by 2i  

作　　者：ShigeoMasuda  MoLi Juan CarlosIzpisua Belmonte 

机构地区：[1]Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA[2]Center for Regenerative Medicine in Barcelona, Dr. Aiguader 88, 08003 Barcelona, Spain

出　　处：《Cell Research》2013年第4期458-459,共2页细胞研究（英文版）

摘　　要：Maintenance of hematopoietic stem cells （HSCs） in vitro has been believed to be difficult due to a lack of complete understanding of HSC quiescence maintained by the niche. Recent evidence suggests that in vitro maintenance of human and mouse long-term HSCs （LT-HSCs） is possible through dual inhibition （2i） of both GSK-3 and mTOR in the absence of cytokines, serum, or feeder cells.

关 键 词：造血干细胞 少维护 饲养层细胞 细胞因子 MTOR HSC 体外 小鼠 

分 类 号：Q813[生物学—生物工程] U448.13[建筑科学—桥梁与隧道工程]