辐照灭菌用于纳豆激酶制剂的实验研究  

The Experiment Study of the γ Radiation on the Nattokinase Crudes

作　　者：周伏忠[1,2] 冯菲[2] 时翔明 孙玉飞[2] 李锋[1] 

机构地区：[1]河南省微生物工程重点实验室,郑州450008 [2]河南省科学院生物研究所有限责任公司,郑州450008 [3]河南省环境保护科学研究院,郑州450004

出　　处：《河南科学》2013年第4期441-444,共4页Henan Science

基　　金：河南省高新技术产业化项目(112105000030)

摘　　要：对60钴γ-射线辐照前后的纳豆激酶(NK)制剂有效成分、酶活及活菌数等技术指标进行了实验研究,结果表明,不同剂量的60钴γ-射线辐照对纳豆激酶制剂中的总皂甙含量和纳豆激酶酶活影响不大,30 kGy时总皂甙含量仍可达到原始含量的105%～125%,NK酶活达到原始酶活的90%以上;纳豆芽孢杆菌、大肠菌群和金黄色葡萄球菌等活菌数对60钴γ-射线辐照十分敏感,5 kGy的60钴γ-射线辐照处理后纳豆芽孢杆菌、大肠菌群和金黄色葡萄球菌数量下降99%以上.The nattokinase etudes were treated with Y-ray irradiation at different absorbed doses, and the total ginsenosides, nattokinase activity and the vital microbes were tested. It was found that the influences of the Y-ray irradiation on the total ginsenosides and nattokinase activity were slightly,while the lethal effect of Y-ray irradiation on the vital microbes such as Bacillus subtilus Natto,Staphylococcus aureus and coliform bacteria were seriously （decreased 99%）. This studies provided some experimental data for irradiation methods applicated on the nattokinase crudes disinfection and preservation.

关 键 词：γ-射线辐照 纳豆激酶酶活 活菌数 灭菌 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] Q814.4[生物学—生物工程]