一种人表皮黑素细胞透射电镜观察方法  

A Method for Transmission Electron Microscopy of Cultured Human Epidermal Melanocyte

作　　者：李伟秋[1] 刘少辉[2] 徐丹丹[2] 方斌[2] 林玲[2] 陈玮莹[2] 

机构地区：[1]汕头大学医学院分析细胞学实验室,广东汕头515041 [2]汕头大学医学院基础医学实验教学中心,广东汕头515041

出　　处：《汕头大学医学院学报》2013年第1期19-20,F0003,共3页Journal of Shantou University Medical College

基　　金：广东省自然科学基金资助项目(9151806001000009);中华医学会-欧莱雅研究资助项目(S2010070806);广东省中医药局资助课题(2010185)

摘　　要：目的:探讨建立人表皮黑素细胞透射电镜标本制备方法。方法:以PET聚酯薄膜为支持物培养人表皮黑素细胞,纵向切片透射电镜观察。结果:人表皮黑素细胞可在PET聚酯薄膜上培养生长,有利于观察人表皮黑素细胞及其树突状突起的超微结构。结论:PET聚酯薄膜可作为支持材料应用于人表皮黑素细胞体外培养,本法可用于多极细胞超微结构的观察。Objective： To establish a sample preparation method for transmission electron microscopy of human epidermal melanocytes.Method：The polyethylene terephthalate film was used as cellular supporting material for culturing of human epidermal melanocytes and the sample for transmission electron microscopy was prepared.Results：The human epidermal melanocytes attached and grown on polyethylene terephthalate film,which was beneficial to ultrastructural observation of human epidermal melanocytes.Conclusion：The polyethylene terephthalate film can be a kind of supporting material for human epidermal melanocytes culturing.The method might be applied to ultrastructure observation of multipolar cells.

关 键 词：人黑素细胞 透射电镜 PET聚酯薄膜 超微结构 

分 类 号：B329.24[哲学宗教—外国哲学] R-33[医药卫生]