胶原蛋白肽增强小鼠免疫力的实验研究  

Collagen peptide enhances immunity in mice

作　　者：李承威[1] 马淑霞[1] 王春敏[1] 任继秋[1] 杨春佳[1] 崔刚[1] 杨景云[1] 吴庆田[1] 

机构地区：[1]佳木斯大学基础医学院微生物教研室,黑龙江佳木斯154007

出　　处：《中国微生态学杂志》2013年第4期387-389,共3页Chinese Journal of Microecology

基　　金：科技部科技支撑项目(2009GJB20034)

摘　　要：目的探讨用生物酶提取的胶原蛋白肽增强小鼠免疫力的作用及相关机制。方法 80只清洁级昆明小鼠被随机分成正常对照组、胶原蛋白肽低剂量组[3 g/(kg.bw)]、中剂量组[7.5 g/(kg.bw)]和高剂量组[12 g/(kg.bw)],每日给药2次,连续2周。检测免疫器官重量、抗体生成细胞数(Jerne改良玻片法)、半数溶血值(HC50)的测定及小鼠腹腔巨噬细胞吞噬鸡红细胞实验。结果高剂量组可提高脾脏指数、胸腺指数、半数溶血值及吞噬细胞吞噬率和吞噬指数、可增加抗体生成细胞数;中剂量组可提高胸腺指数、增加抗体生成细胞数、提高小鼠半数溶血值及吞噬细胞吞噬率和吞噬指数;低剂量组可增加抗体生成细胞数、提高吞噬细胞的吞噬率和吞噬指数。结论三种剂量胶原蛋白肽对正常小鼠的细胞免疫、体液免疫均有显著的增强作用,高剂量组效果最强。Objective To discuss the role and mechanisms of biological enzyme-extracted collagen peptide enhancing mice's immunity.Methods 80 clean Kunming mice were randomly divided into normal control group(given saline),low-dose collagen peptide group [3 g/(kg·bw)],medium-dose group [7.5 g/(kg·bw)] and high-dose group [12 g/(kg·bw)].Collagen peptide was given twice daily for 2 consecutive weeks.The immune organ weight,number of antibody-producing cells(Jerne improved slide method),50% hemolysin value(HC50) and macrophage phagocytosis of chicken erythrocytes were detected.Results The high dose group showed increased thymic index,splenic index,50% hemolysin value,rate of phagocytosis and phagocytic index,as well as increased number of antibody-producing cells;The medium dose group showed increased thymic index,number of antibody producing cells,50% hemolysin value,rate of phagocytosis and phagocytic index;The low dose group showed increased number of antibody producing cells,rate of phagocytosis and phagocytic index.Conclusion Collagen peptide can enhance the cellular immunity and humoral immunity significantly in normal mice;high dose results in better effect.

关 键 词：胶原蛋白肽 生物酶 免疫系统 

分 类 号：R3[医药卫生—基础医学]