在淡豆豉发酵过程中桑叶、青蒿对大豆异黄酮含量的影响  

Effects of Mori folium and Artemisiae annuae herba on isoflavone contents in the course of Sojae semen praeparatum fermentation

作　　者：张敏敏[1] 廖丽娜[1] 曹尉尉[2] 毛峻琴[3] 

机构地区：[1]安徽中医学院药学院研究生部,合肥230038 [2]解放军第411医院药剂科,上海200081 [3]解放军第85医院药剂科,上海200053

出　　处：《药学服务与研究》2013年第2期119-123,共5页Pharmaceutical Care and Research

基　　金：上海市卫生局中医药科研基金(2010Y006A)

摘　　要：目的:研究在淡豆豉发酵过程中桑叶、青蒿对大豆异黄酮含量的影响。方法:样品分4组:每组均含黑大豆300g,组2含青蒿30g,组3含桑叶30g,组4含青蒿、桑叶各30g。发酵21d。采用HPLC法测定大豆异黄酮含量。色谱柱:Diamonsil C18柱(250mm×4.6mm,5μm),流动相:乙腈-3%冰乙酸,梯度洗脱,流速:1ml/min,柱温:30℃,检测波长:261nm。测定发酵过程中大豆苷、染料木苷、大豆苷元、染料木素的含量变化。结果:随着发酵时间延长,各组大豆苷、染料木苷含量呈逐步下降趋势,苷元含量呈增加趋势,12d后变化趋于稳定。组4的大豆苷、染料木苷含量显著低于组2、3(P<0.01),多数大豆苷元、染料木素含量显著高于组2、3(P<0.01)。结论:在淡豆豉发酵过程,桑叶、青蒿可以促进大豆异黄酮苷向苷元的转化。Objective： To study the effects of Mori folium and Artemisiae annua herba on isoflavone contents in the course of Sojae semen praeparatum （SSP） fermentation. Methods： The test samples were divided into 4 groups：group 1,2,3 and 4, with each group containing 300 g of black soybean, group 2 containing 30 g of Artemisiae, group 3 containing 30 g of Mori folium,group 4 containing 30 g of Artemisiae and 30 g of Mori folium. Then, all the sample groups were fermented for 21 days at preset conditions. HPLC method was performed to detect the content of isoflavone. Chromatographic column：Diamonsil C18 column （250 mm× 4.6 mm, 5 μm）; mobile phase of acetonitrile 3% glacial acetic acid; gradient elution at a flow rate of 1.0 ml/min; column temperature of 30 C ;and detection wavelength of 261 nm. Then,changes in the contents of daidzin,genistin,daidzein and genistein were detected in the course of fermentation. Results： With prolonged fermentation of SSP, the contents of daidzin and genistin in all the groups gradually decreased, while the content of aglycone tended to increase. After 12 days, changes in their contents tended to be stable. The contents of daidzin and genistin in group 4 were significantly lower than that of group 2 and 3 （P〈0.01）, while the contents of daidzein and genistein in group 4 were significantly higher than group 2 and 3 （P〈0.01）, when comparisons were made between the 4 groups. Conclusion： In the course of SSP fermentation, Mori folium and Artemisiae could promote the transformation of glycosides into daidzein.

关 键 词：淡豆豉 异黄酮 桑叶 青蒿 发酵 色谱法 高效液相 

分 类 号：R284[医药卫生—中药学]