1,3-二氯-2-丙醇对睾丸间质细胞R2C活性及孕酮合成的影响  

Effect of 1,3-Dichloro-2-propanol on Cell Growth and Progesterone Biosynthesis of R2C Mouse Leydig Cells

作　　者：白顺[1] 孙建霞[2] 白卫滨[3] 邹飞雁[1] 张齐好[4] 黄亚东[4] 

机构地区：[1]暨南大学发育与再生生物学系,广东广州510632 [2]广东工业大学轻工化工学院,广东广州510006 [3]暨南大学食品科学与工程系,广东广州510632 [4]暨南大学医药生物技术研究开发中心,广东广州510632

出　　处：《食品科学》2013年第9期292-295,共4页Food Science

基　　金：国家自然科学基金项目(31201340);"十二五"国家科技支撑计划项目(2012BAK01B00)

摘　　要：目的:研究1,3-二氯-2-丙醇(1,3-DCP)对体外睾丸间质细胞活性及孕酮合成的影响。方法:分别用0.5、1、2、4、6mmol/L浓度的1,3-DCP作用R2C细胞48h,利用MTT法获得1,3-DCP对R2C细胞的IC25、IC50以及IC75。选取以上3种不同浓度的1,3-DCP作用细胞4h或24h,通过单细胞电泳法(SCGE)检测其作用4h时对DNA损伤的程度;通过放射免疫法(RIA)检测4h或24h处理时对孕酮合成的影响。结果:与对照组相比,1,3-DCP能抑制R2C细胞的增殖,其IC25、IC50、IC75分别为(1.161±0.046)、(1.897±0.018)、(3.100±0.040)mmol/L;作用4h对R2C细胞DNA有损伤作用;各浓度组作用4h或24h后与对照组相比均能降低孕酮的合成。结论:1,3-DCP能抑制R2C的活性,影响细胞孕酮的合成。Objective： To explore the effect of 1,3-dichloro-2-propanol（1,3-DCP） on cell growth and progesterone biosynthesis in R2C mouse leydig cells.Methods： R2C cells were exposed to 0.5,1,2,4 mmol/L,and 6 mmol/L 1,3-DCP for 48 h,different concentrations（IC25,IC50and IC75） of 1,3-DCP were elected by MTT assay.R2C cells were stimulated by three concentrations for 4 h or 24 h,respectively.The damage of DNA in R2C cells was investigated by single cell gel electrophoresis（SCGE）.Progesterone level in supernatant of cell culture medium was measured by radioimmunoassay（RIA）.Results： 1,3-DCP could inhibit cell growth,and IC25,IC50and IC75values were（1.161 ± 0.046）,（1.897 ± 0.018） mmol/L and（3.100 ± 0.040） mmol/L.In comparison with the control group,the damage of DNA were obviously higher in three concentrations of 1,3-DCP for 4 h,and the progesterone level were also obviously lower in three concentrations of 1,3-DCP for 4 h or 24 h.Conclusion： 1,3-DCP can directly affect cell growth and the progesterone synthesis of R2C cells.

关 键 词：1 3-二氯-2-丙醇 睾丸间质细胞 孕酮 细胞活性 机制 

分 类 号：TS201.6[轻工技术与工程—食品科学]