高脂饮食和病毒感染损伤血管内膜组织的比较研究  

Comparison the Difference of Intima Injurys Induced by Human Cytomegalovirus and Hyperlipemia in Rabbits

作　　者：孟红[1] 王健[1] 刘菊华[1] 孙广莲[1] 李焱[1] 张家驹[1] 张卫东[1] 

机构地区：[1]山东省医学科学院基础医学研究所微生物室,山东省济南市250062

出　　处：《中国动脉硬化杂志》2000年第3期213-216,共4页Chinese Journal of Arteriosclerosis

基　　金：山东省自然科学研究基金!资助课题 :Y96C 16 0 48

摘　　要：为了比较病毒感染与高脂饮食造成家兔的血管组织病理变化。将 6 0只家兔分为对照组 (正常对照组 )、灭活病毒组、高脂饮食组、病毒感染组四组 ,随后给予相应处理。 6 0d后取动物血管组织 ,以免疫组织化学方法检测组织内病毒抗原作为病毒感染的证据 ,分析高脂饮食与病毒感染损伤血管内膜的光镜和电镜病理学特点。结果发现 ,对照组动物血管组织无论光镜或电镜均未见明显异常。高脂组动物光镜下在血管查到脂质沉积 ,红细胞粘附 ;扫描电镜可见内皮细胞破损 ,有红细胞和白细胞粘附于内皮细胞表面 ;透射电镜可见动物血管内皮细胞、平滑肌细胞和巨噬细胞内有脂滴。在病毒组动物 ,光镜检查发现血管组织损伤主要为内皮细胞的变性、坏死和脱落 ,部分血管腔内有密度较高的呈丝状、颗粒状似“血栓样堆积物” ;扫描电镜发现内皮细胞表面有丝状物粘附 ;透射电镜检查发现血管基底膜增宽 ,厚度不均 ,内皮细胞微突增多 ,核呈分叶状 ,异染色质积聚 ,靠核膜处电子密度增高 ,核膜呈皱折状。在一份血管标本的平滑肌细胞浆内查到可疑病毒颗粒 ;免疫组织化学法在多组织细胞、尤其是血管内皮细胞查到人巨细胞病毒抗原。实验结果提示 ,病毒感染与高脂饮食造成的血管内皮细胞损伤病理改变不同。Aim To compare the difference between human cytomegalovirus (hCMV)-induced and hyperlipemia-induced in rabbits. Methods The rabbits were randomly divided into three groups: the control group, the group fed on diet containing high lipid, and the group infected with hCMV AD169 strain. In 60th day the arterial tissue speciments were detected by immunohistochemistry, pathological method and electron microscopy. Results The pathological alterations of the arterial intima of the rabbits inoculated with hCMV, were EC swelling, degeneration, necrosis and dropping from intima with adhering fibrin-like materials. Four thrombus-like materials were found in nineteen of arterial speciments. The hCMV antigens were detected in EC and virion-like materials were found in smooth muscle cells. Fibrin-like materials adhering to the surface of ECs were manifested by electron microscopy;The pathological alterations of the arterial intima of the rabbits which fed on diet containing high lipid were atherosclerosis. A large number of red blood cells adhering to the surface of the EC were found by electron microscopy. Conclusion There are some differences in the pathological changes of the intimal injury between the hCMV-induced and hyperlipemia-induced of in Rabbits.

关 键 词：高脂饮食 巨细胞病毒 感染 家兔 损伤 内皮 血管 

分 类 号：R365[医药卫生—病理学]