黄芩苷和重组人骨形成蛋白2双缓释制剂促进小型猪牙周组织再生的初步研究  

Preliminary study of the dual release baicalin and rhBMP-2 system to improve periodontal tissue regeneration in minipigs

作　　者：曾辉[1] 李凤[1] 魏虹[2] 石建峰[2] 饶国洲[2] 李昂[2] 苟建重[1] 

机构地区：[1]西安交通大学口腔医学院牙周黏膜病科,陕西西安710004 [2]西安交通大学口腔医学院口腔医学研究中心,陕西西安710004

出　　处：《上海口腔医学》2013年第2期126-131,共6页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81173311);陕西省"13115"科技创新工程重大科技专项项目(2009ZDKG-77)~~

摘　　要：目的:研究黄芩苷和重组人骨形成蛋白2双缓释制剂联合应用对小型猪牙周组织的再生作用。方法:建立小型猪牙周炎动物模型,随机分为4组。双缓组——壳聚糖温敏凝胶+重组人骨形成蛋白液(rhBMP-2)+黄芩苷-明胶微球(GMS),单黄组——壳聚糖温敏凝胶+黄芩苷-GMS,BMP组——壳聚糖温敏凝胶+rhBMP-2液,阴性对照组——空白凝胶。在建模前、后6周,治疗后4周和8周检查牙周病的相关临床指标,龈沟液IL-1β、TNF-α的变化。采用SPSS13.0软件包对数据进行统计学分析,并于治疗后8周进行组织学观察。结果:(1)各组在治疗4周、8周后,临床检查指标及龈沟液IL-1β、TNF-α检查水平与建模6周相比均有显著差异(P<0.05);双缓组治疗效果优于其他各组(P<0.05),且各项指标呈持续改善状态。X线片显示,治疗8周后,与对照组相比,其他3组骨密度及牙槽骨高度都有所增加,其中,双缓组骨密度及高度增加最为显著。(2)组织学改变:治疗8周后,阴性对照组牙周组织炎症反应明显,无明显修复反应;其他3组可见不同量的新生骨质。其中,双缓组牙周组织炎症程度较对照组明显减轻,修复反应最为明显。结论:黄芩苷和rhBMP-2联合应用能更好地促进牙周组织再生,为黄芩苷和rhBMP-2双缓释体系的临床应用奠定了基础。PURPOSE： To explore the feasibility of dual release baicalin and rhBMP-2 system for the treatment of periodontal diseases in minipigs. METHODS： Four miniature swines were selected to establish the model of periodontitis and randomly divided them into four groups： Dual release group, hydrogel with baicalin-GMS and rhBMP-2; Single Huang group, hydrogel with baicalin-GMS; BMP group, hydrogel with rhBMP-2; Negative control group, blank hydrogel. All parameters including clinical indicators of periodontitis, the level of IL-1 and TNF-α in the gingival erevicular fluid （GCF） were measured before and 6 weeks after modeling, and 4, 8 weeks after treatment. The data was analyzed using SPSS13.0 software package. The periodontitis model animals were sacrificed at the end of 8 weeks after treatment. The histological changes of periodontal tissues were observed under microscope with HE staining. RESULTS： （1）In all groups, there were significant differences of clinical indicators and levels of IL-1 and TNF-α in the GCF between periodontitis was established and 4, 8 weeks after treatment （P〈0.05）. Dual release group was significantly superior than that of other three groups （P〈0.05）. Moreover, all the parameters improved continuously. X-ray showed that bone mineral density and height in the remaining three groups increased compared with the control group, with highest in dual release group. （2）The results of histological observation showed that the inflammation reaction of periodontal tissues in negative control group was more serious than that in other groups 8 weeks after treatment. There was no obvious repair reaction in the control group, but different amount of new bone was seen in other three groups. Among them, dual release group had most obvious repair reaction. CONCLUSIONS： The dual-slow-release chitosan thermosensitive hydrogel system is employable to be used effectively in the regeneration of periodontal defects, which laid a foundation for further clinical use. Supported by Nation

关 键 词：黄芩苷 RHBMP-2 小型猪 牙周炎 

分 类 号：R781.4[医药卫生—口腔医学]