狗牙根糖化液发酵制备乙醇的工艺研究  

Study on the technology of production of fermented ethanol from Bermuda grass's saccharification liquid

作　　者：刘俊红[1] 王福梅[1] 程慧[1] 赵奇[1] 

机构地区：[1]河南城建学院生物工程系,河南平顶山467044

出　　处：《可再生能源》2013年第5期84-89,共6页Renewable Energy Resources

基　　金：河南省科技厅基础与前沿项目(092300410083)

摘　　要：禾本科狗尾草属植物——狗牙根,纤维素含量非常丰富,纤维素经过稀酸、纤维素酶和木聚糖酶处理后,接种嗜鞣管囊酵母发酵可得到生物乙醇。试验以乙醇得率为指标,研究了葡萄糖、酵母膏、蛋白胨、接种量、温度、时间、pH等因素对乙醇得率的影响,通过正交试验,得到最佳的试验组合,最后用狗牙根糖化液代替葡萄糖进行发酵,生产制备生物乙醇。结果表明,在以葡萄糖为原料的乙醇生产过程中的最佳条件是:100 mL的培养基中,葡萄糖20 g、酵母膏1.5 g、蛋白胨0.5 g、接种量13 mL、温度30℃、时间60 h、pH5.5、硫酸铵1 g、磷酸二氢钾0.2 g、硫酸镁0.1 g,乙醇的最高产率为43.35%;狗牙根糖化液最终乙醇得率为7.04%。Bermuda grass, rich in cellulose content, is an annual vegetation of gramineous grass family. After the processing of dilute acid, cellulose and xylanase, cellulose can be decomposed, which can be fermented by pachysolen tannophilus, then bioethanol is obtained. Taking ethanol yield as objective, the experiment studies the effects of single factors on ethanol yield, glucose content, yeast extract content, peptone content, inoculation quantity, temperature, time and pH value are all included. Then by the orthogonal experiment, the optimal experiment combination is chosen. Finally, replace glucose with saccharification liquid of bermuda grass for fermentation. The results showed that the optimum production conditions of using glucose as the raw material is as followed. In 100 mL culture medium, glucose 20 g, yeast extract 1.5 g, peptone 0.5 g, inoculation quantity 13 mL, temperature of 30 ℃, time 60 h, pHS.5, ammonium sulfate lg, potassium dihydrogen phosphate 0.2 g, magnesium sulfate 0.1 g, the ethanol yield is 43.35%, that of bermuda grass saccharification liquid is 7.04%.

关 键 词：纤维素 狗牙根 糖化液 发酵 乙醇 

分 类 号：TK6[动力工程及工程热物理—生物能] S216.2[农业科学—农业机械化工程]