不同培养基对毕赤酵母工程菌生长的影响  

The Effect that Different Culture Medium Give to the Growth of Pichia pastoris Yeast Strain

作　　者：赵炎葱[1] 李景华[2] 赵学礼[1] 

机构地区：[1]河南大学第一附属医院检验科,开封475001 [2]河南大学天然药物与免疫工程重点实验室

出　　处：《中国医疗前沿》2013年第7期35-36,共2页China Healthcare Innovation

基　　金：河南大学科学研究基金(编号:2010YBZR00)

摘　　要：目的确定接种量对重组Pichia pastoris GS115Muts菌株的影响和更适合目的菌株的培养基。方法采用三种不同的培养基(YPD培养基、BMGY培养基和复合培养基)培养重组Pichia pastoris GS115Muts菌株,以1.0%、1.5%、2.0%的接种量接种,分别在4h和24h的时间间隔下,采用比浊法对酵母菌96h内的生长进行OD值测定。结果复合培养基和YPD培养基是三种培养基较为合适的,且在12-24h之间是重组酵母菌的对数生长期,此时是接种的最佳时间,接种量对菌株的生长没有明显影响。结论合适的培养基和适当的接种时间对巴斯德毕赤酵母(Pichia pastoris)的培养关系密切。Objectice This Experiment is testing the most suitable culture medium and the right time when the seed liquid is most applicable. Methods There are three kinds of culture medium in this experiment-YPD, BMGY, and compound culture medium. They are used to reform a new strain named Pichia pastoris GSllSMuts. The OD is tested every 4 and 24 hours, within 96 hours, in the way of turbidimetric method. Result The compound culture medium and the YPD are the better culture medium in three mediums. And the best time to vaccinate is the period between 12 and 24 hours. Conclusion Pasteur Pichia yeast Strain is closely related to the Suitable medium and appropriate inoculation time.

关 键 词：毕赤酵母 培养基 发酵 

分 类 号：R392.3[医药卫生—免疫学]