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机构地区:[1]湖北师范学院生命科学学院,湖北黄石435002
出 处:《微生物学杂志》2013年第2期28-32,共5页Journal of Microbiology
基 金:国家高技术研究发展计划项目(2009ZX07104-005-02);国家重点实验室开放基金(2009FB10);湖北省教育厅重点项目(D20092202)
摘 要:分别测量培养在不同氮浓度培养基中的葛仙米叶绿素a含量和3种叶绿素荧光参数(Fv/Fm、PSⅡ、ETR),研究外加氮源对葛仙米生长和光合生理的影响。结果表明:外加氮源抑制葛仙米的生长,并且随着浓度升高,抑制效果更加明显。外加氮源浓度小于等于1.5 g/L时,葛仙米光合电子传递速率(ETR)、PSⅡ最大量子产量(Fv/Fm)和PSⅡ光化学反应量子效率(PSⅡ)值均有先下降后上升趋势;而当外加氮源浓度大于1.5 g/L时,上述3种荧光参数值先维持不变后有下降趋势。Effects of additional nitrogen resources on the growth and photosynthesis physiology of Nostoc sphaeroids were studied by culturing it in the medium with different nitrogen concentrations, and then content of chlorophyll a and three types of chlorophyll fluorescence parameters (Fv/Fm, ФPSⅡ , ETR) were measured. The results indicated that additional nitrogen resources inhibited the growth of N. sphaeroides, and with the increase of nitrogen concentration, the inhibitory effect was more obvious. When the additional nitrogen concentration was ≤ 1.5 g/L, N. sphaeroides photosynthetic electron transport rate (ETR), maximum quantum yield of PS Ⅱ (Fv/Fm) and PS Ⅱ photochemical quantum efficiency ( ФPS Ⅱ ) values were decreased first and then increased. When the nitrogen concentration was 〉 1.5 g/L, the above three kinds of fluorescence parameters kept unchanged first and then showed a downward trend.
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