利用木糖高产乙醇酵母菌发酵条件的研究  

Study on the Fermentation Conditions of Xylose by Pichia guilliermondii to Produce Ethanol

作　　者：赖颖[1] 李青芝[1] 赵锦慧[1] 

机构地区：[1]周口师范学院,河南周口466001

出　　处：《酿酒科技》2013年第5期31-34,共4页Liquor-Making Science & Technology

基　　金：周口师范学院青年科研基金项目(zknuqn201125B)

摘　　要：以蒙假丝酵母JS-73为出发菌株,逐渐增加底物中木糖浓度,对该菌株利用木糖产乙醇的能力进行了驯化,并优化该菌利用木糖生产乙醇的工艺和培养基成分。结果表明,驯化后,木糖利用率为67.21%,酒精产量达到7.41 g/L。确定了发酵培养基的最优组合为:木糖60 g/L,酵母粉0.6 g/L,K2HPO41.0 g/L,MgSO4.7H2O 0.4 g/L,在该条件组合下,菌株JS-73的乙醇产量为10.27 g/L,是理论产量的37.21%。确定了发酵最优条件为:温度31℃,pH5,转速70 r/min,接种量11%,装液量120 mL/250 mL,在此条件下,乙醇产量达最高,为18.95 g/L。In the experiment, Pichia guilliermondii JS-73 was used as the starting strain and then cultured to produce ethanol with increasing xy- lose content gradually in the substrate. The results indicated that the utilization rate of xylose was 67.2 1% and the yield of ethanol was 7.41 g/L. Furthermore, the culture mediums had been optimized and it was composed of 60 g/L xylose, 0.6 g/L yeast powder, 1.0 g/L K2HPO4 and 0.4 g/L MgSO4-7H20. In these conditions, ethanol yield of strain JS-73 reached up 10.27 g/L, 37.21% of theoretical yield. The best fermenting conditions had been determined as follows： temperature at , pH was 5, rotating speed was 70 r/min, inoculating quantity was 11%, and liquid filling quantity was 120 mL/250 mL. In these conditions, the yield of ethanol reached up the highest as 18.95 g/L.

关 键 词：木糖 乙醇 发酵 季也蒙假丝酵母 

分 类 号：TQ223.122[化学工程—有机化工]