气相色谱-同位素质谱(GC-C-IRMS)测定饮料酒中乙醇^(13)C/^(12)C比值方法研究  

Study on the Determination of ^(13)C/^(12)C of Ethanol in Alcoholic Beverage by GC-C-IRMS

作　　者：陈文斌[1,2] 钟其顶[2,3] 王道兵[2] 陶宏兵[1,2] 武竹英[2] 熊正河[2] 蒙健宗[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530004 [2]中国食品发酵工业研究院,北京100027 [3]天津科技大学,天津300457

出　　处：《酿酒科技》2013年第5期90-93,共4页Liquor-Making Science & Technology

基　　金：科技部国际合作项目(2011DFA33270);国家自然基金委(31101333);科技部公益项目(201210104)

摘　　要：建立了以有机溶剂稀释法为前处理方法测定饮料酒中乙醇碳稳定同位素比率的气相色谱-燃烧-稳定同位素比值质谱法(GC-C-IRMS)。通过条件优化,在将乙醇稀释至8 g/L并采用Wax色谱柱进行有效分离后,可实现对乙醇中碳同位素比值的有效测定。结果表明,该法操作简便,稳定性好,准确度高,可为通过乙醇同位素比值差异实现饮料酒的真实性鉴别提供技术方法。GC-C-IRMS with the pretreatment of dilution by organic solvent had been developed to determine 13C/l2C of ethanol in alcoholic beverage. Firstly, ethanol in alcoholic beverage was diluted to 8 g/L and then separated by Wax chromatographic column under optimized conditions, and then carbon isotopic ratio of ethanol could be measured. The results proved that such method was simple with high accuracy and good repro ducibility. The identification of alcoholic beverage authenticity could be carded out through the comparison of the difference in 13C/12C of ethanol in practice.

关 键 词：饮料酒 乙醇 碳同位素 δ13C IRMS 

分 类 号：O657.63[理学—分析化学] TS207.3[理学—化学]