柱前衍生RP-HPLC法分析淡豆豉和大豆中氨基酸  

Analysis on amino acids in Sojae Semen Preparatrm and Glycine max by pre-column derivatization RP-HPLC

作　　者：李萍萍[1] 崔元璐[1] 蒋庆峰[2] 

机构地区：[1]天津市现代中药重点实验室,天津中医药大学,天津300193 [2]天津药物研究院,天津300193

出　　处：《中草药》2013年第9期1199-1202,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81173469);教育部新世纪优秀人才资助项目(NCET-09-0899)

摘　　要：目的分析淡豆豉Sojae Semen Preparatum和大豆Glycine max中游离及水解氨基酸。方法用0.1 mol/L HCl作溶剂超声提取得到淡豆豉和大豆中的游离氨基酸;用6 mol/L HCl水解提取得到水解氨基酸,以异硫氰酸苯酯(PITC)为柱前衍生化试剂,采用柱前衍生RP-HPLC法进行分析。结果淡豆豉和大豆中均含有16种氨基酸,这16种氨基酸浓度在0.031～1.750 mmol/L内呈良好的线性关系,r均大于0.997 9,平均回收率91.02%～102.04%,RSD1.01%～4.81%。结论本方法灵敏、准确,具有良好的重复性和稳定性,16种氨基酸能在样品杂质量较多的情况下得到分离,且采用普通C18色谱柱成本低,可广泛应用于富含氨基酸样品的分析。Objective To analyze the free and hydrolyzed amino acids in Sojae Semen Preparatum （SSP） and Glycine max （GM） by pre-column derivatization RP-HPLC. Methods Free amino acids extracted in HC1 （0.1 mol/L） by ultrasonic method and hydrolyzed amino acids extracted in HC1 （6 mol/L） by hydrolysis method were both derivated by the agent phenylisothiocyanate so as to analyze them by gradient elution. Results There were 16 kinds of amino acids in both SSP and GM. The 16 kinds of amino acids had good linearity in the range of 0.031-1.750 pmol/mL with the coefficients of correlation all over 0.9979. The average recoveries were 91.02%-102.04% and the RSD values were between 1.01% and 4.81%. Conclusion The method is not only sensitive and accurate, but also has the high repeatability and stability, and the 16 kinds of amino acids could be separated in the case of the samples with more impurities. The common C18 column is widely used for the determination of many other samples fiched in amino acids at lower cost.

关 键 词：氨基酸 淡豆豉 大豆 异硫氰酸苯酯 柱前衍生高效液相色谱 

分 类 号：R286.022[医药卫生—中药学]