非小细胞肺癌患者外周血c-Cbl基因突变研究  

c-Cbl gene mutation in peripheral blood from patients with non-small-cell lung cancer

作　　者：冯士生 梁建琴[2] 王金河[2] 史晓朋[2] 

机构地区：[1]解放军医学院研究生队,北京100853 [2]解放军第309医院全军结核病研究所结核二科,北京100091

出　　处：《中国临床医生杂志》2013年第5期29-32,共4页Chinese Journal For Clinicians

摘　　要：目的检测非小细胞肺癌(NSCLC)患者外周血c-Cbl基因突变的情况。方法收集20例正常健康人、20例肺结核患者和40例NSCLC患者外周血标本,采用RT-PCR扩增和基因测序方法,检测c-Cbl基因突变情况。结果 c-Cbl在正常健康人及肺结核患者外周血中均未检出基因突变;NSCLC患者外周血中检测到4种c-Cbl基因突变,外周血中检出c-Cbl基因的突变率为20%(8/40);c-Cbl基因突变率与患者的性别,年龄及病理学分型均无相关性(P>0.05);而不同临床分期间c-Cbl基因突变率不同,差异有显著性(P<0.05)。结论 NSCLC患者外周血中检测到c-Cbl基因突变,且发现不同临床分期间突变率不同。Objective In order to investigate c-Cbl Gene mutations in peripheral blood from non-small cell lung canc-er （NSCLC） patients. Method The peripheral blood from 40 cases of NSCLC, 20 cases of normal patients and 20 cases of TB patients were selected. RNA was extracted, then the c-Cbl gene was subjected by RT-PCR and direct se-quencing. The statistical analysis was performed by using the SPSS programs. Result c-Cbl gene mutations were not detected in peripheral blood from all the selected normal patients and TB patients. Four kinds of c-Cbl gene muta-tions were found in 8 cases of the 40 NSCLC patients, with an incidence of 20%. The mutation rate ofc-Cbl do not related to gender, age and pathomorphological types （P〉0.05） ; but related to clinical stage closely （P〈0.05）. Conclusion The c-Cbl gene mutations were detected in peripheral blood from NSCLC. What＇ s more, it was found that the mutation rate of c-Cbl related to clinical stage closely.

关 键 词：非小细胞肺癌 c—Cbl 基因突变 外周血 

分 类 号：R734.2[医药卫生—肿瘤]