微生物降解聚乳酸生产乳酸的研究  

Research on Production of Lactic Acid by Biodegrading Poly(L-Lactic Acid)

作　　者：高佳[1] 李琳琳[1] 王战勇[1] 

机构地区：[1]辽宁石油化工大学环境与生物工程学院,辽宁抚顺113001

出　　处：《科技通报》2013年第5期173-176,共4页Bulletin of Science and Technology

基　　金：国家自然科学基金项目(31100099);辽宁省教育厅科学研究项目(L2011060)

摘　　要：利用聚乳酸降解菌门多萨假单胞菌DS04-T降解聚乳酸生成乳酸单体。结合单因素考察和正交试验,优选出适于菌株DS04-T降解聚乳酸生产乳酸的最适条件:将种子培养液按6%(V/V)接种量接种于装有100 mL PLA培养基(pH 8.0)的250 mL三角瓶中,37°C,160 r/min振荡培养3 d。此条件下获取的乳酸产量为(511±12)mmol/L。Strain Pseudomonas mendocina DS04-T was used to degrade PLA [poly(L-Lactic Acid)] for producing L-Lactic Acid.According to single factor influence and orthogonal test,the optimized production conditions were obtained as follows: the 6%(V/V) seeds were inoculated in 250 mL triangular flask with 100mL PLA culture medium(pH 8.0) at 37°C for 3 days when rotating at 160 r/min.Under the condition,the yield of L-Lactic Acid reached 511±12 mmol/L.

关 键 词：聚乳酸 门多萨假单胞菌 降解 乳酸 

分 类 号：X172[环境科学与工程—环境科学]