黄曲霉毒素M_1化学发光酶免疫检测方法的建立  

Development of chemiluminescence enzyme immunoassay for the determination of aflatoxin M_1

作　　者：王岩[1] 于源华[1] 王维[1] 乔玉龙[1] 周帅[1] 孙立志[1] 

机构地区：[1]长春理工大学吉林省生物检测工程实验室,长春130022

出　　处：《食品科技》2013年第5期342-346,共5页Food Science and Technology

基　　金：吉林省科技厅重大科技攻关项目(10ZDGG005)

摘　　要：建立黄曲霉毒素M1的直接竞争化学发光酶免疫分析（CLEIA）快速检测方法。所建立的方法分析检出限为0.00235ng/mL,检测线性范围为0.00604~1.7326ng/mL,IC50为0.01023ng/mL。批内变异系数小于2.655%,批间变异系数小于6.0175%,牛奶样品添加回收率平均达到97.20%,奶粉样品添加回收率平均达到97.69%。该方法简单、灵敏、快速,适用于乳制品中黄曲霉毒素M1的检测。A direct competitive chemiluminescence enzyme immunoassay （CLEIA） was developed for rapidly determining aflatoxin M1.The optimized CLEIA gave a limit detection of 0.00235 ng/mL and a detection range of 0.00604～1.7326 ng/mL,with an IC50 of 0.01023 ng/mL.It has been validated in milk samples in terms of precision （intra and interassay coefficient variations of less than 2.655% and 6.0175%,respectively） and accuracy （mean recovery 97.20% in milk and 97.69% in milk powder）.The assay was sensitive,fast and accurate,which proved to be suitable for the screening of milk samples for the presence of AFM1.

关 键 词：黄曲霉毒素M1 化学发光酶免疫分析法 检测 

分 类 号：TS207.5[轻工技术与工程—食品科学]