气相色谱-质谱联用法测定烟草及烟草制品中的麦角甾醇  

Determination of Ergosterol in Tobacco and Tobacco Products with Gas Chromatography/Mass Spectrometry

作　　者：戚大伟[1] 林华清[1] 沙云菲[1] 吴达[1] 刘百战[1] 

机构地区：[1]上海烟草集团有限责任公司技术中心,上海市杨浦区长阳路717号200082

出　　处：《烟草科技》2013年第5期41-45,共5页Tobacco Science & Technology

基　　金：国家烟草专卖局标准项目"烟草及烟草制品霉变的测定方法"(国科烟[2011]355号)

摘　　要：为准确测定烟草及烟草制品中麦角甾醇的含量,建立了以7-去氢胆固醇为内标,将麦角甾醇和7-去氢胆固醇衍生后进行GC/MS同步选择离子检测/全扫描的定量检测方法,并采用该法测定了不同种类烟叶及卷烟中的麦角甾醇含量。结果表明:该方法在2.0～200.0μg/mL之间具有良好的线性关系,R2=0.9995。方法的检出限和检测限分别为0.46和1.53 mg/kg,回收率在85.6%～91.3%之间。该方法适用于烟草及烟草制品中麦角甾醇的定量检测。In order to accurately determine the ergosterol in tobacco and tobacco products, a quantitative determination method by GC/MS with selected ion monitoring/full scan mode was established by taking 7-dehydrocholesterol as an internal standard and derivatizing before GC/MS analysis, and the contents of ergosterol in tobacco leaves and cigarettes of different kinds were determined. The results showed that the calibration curve exhibited good linearity with a correlation coefficient of 0.9995 in the range of 2.0 to 200.0 μg/mL. The LOD and LOQ were 0.46 and 1.53 mg/kg, respectively. The recovery ranged from 85.6% to 91.3%. This method is suitable for the quantitative determination of ergosterol in tobacco and tobacco products.

关 键 词：烟草 烟草制品 霉菌污染标志物 麦角甾醇 7-去氢胆固醇 GC MS 

分 类 号：TS411[农业科学—烟草工业]