固相萃取-高效液相色谱-可变波长检测法同时测定调味品中甜味剂和人工合成色素  

Simultaneous determination of saccharin sodium and synthetic colours in flavourings bysolid phase extraction and highperformance liquid chromatography

作　　者：赵飞[1] 高广慧[1] 那海秋[1] 马殿君[1] 王晓黎[1] 

机构地区：[1]辽宁省食品药品检验所,辽宁沈阳110023

出　　处：《色谱》2013年第5期490-493,共4页Chinese Journal of Chromatography

摘　　要：建立了同时测定调味品中糖精钠和合成色素的固相萃取－高效液相色谱（SPE-HPLC）分析方法。样品经石油醚低温去除油脂，聚酰胺固相萃取柱净化和富集，然后进行HPLC分析。采用反相C18色谱柱，以甲醇和0．02mol／L醋酸铵为流动相进行梯度洗脱，使用可变波长分别在230、510、484和510nm进行检测。实验结果表明：糖精钠与合成色素的分离效果良好．回收率为88．6％～97．1％，相对标准偏差小于5％；方法检出限均可达到0．05mg／kg。该方法操作简单，结果准确可靠，重现性好，适用于大批量调味品中甜味剂和人工合成色素的同时检测。A method for the simultaneous determination of saccharin sodium and synthetic col- ours in flavourings by solid phase extraction （SPE） and high performance liquid chromatogra- phy with variable wavelengths was established. After degreased with petroleum ether, extrac- ted, purified with SPE, and concentrated, the sample was separated on a C,s column with a gradient elution of 0.02 mol/L ammonium acetate and methanol, and detected at 230, 510, 484 and 510 nm, separately. The saccharin sodium and synthetic colours were separated with this method. The recoveries were 88.6% -97. 1% with the RSD 〈 5%. The limit of detection was 0.05 mg/kg for all the analytes. The proposed method is simple, reliable and reproducible, and especially suitable for a lot of sample detections of saccharin sodium and synthetic colours in flavourings.

关 键 词：固相萃取 高效液相色谱法 糖精钠 合成色素 调味品 

分 类 号：O658[理学—分析化学]