热休克蛋白70、热休克蛋白90基因在急性淋巴细胞白血病骨髓单个核细胞的表达及其与糖皮质激素抵抗的关系  

Relationship analysis of the expressions of heat shock protein 70 and heat shock protein 90 genes in bone marrow mononuclear cells with glucocorticoid-resistence in acute lymphoblastic leukemia pa-tients

作　　者：李颖[1] 刘冀衡 刘竞[1] 

机构地区：[1]中南大学湘雅三医院血液科,长沙410013 [2]长沙市一医院血液肿瘤科

出　　处：《中国医师杂志》2013年第4期486-489,共4页Journal of Chinese Physician

摘　　要：目的探讨HSP70、HsPg0基因表达与急性淋巴细胞白血病（ALL）糖皮质激素抵抗关系。方法初诊急性淋巴细胞白血病患者43例，均接受泼尼松治疗。根据疗效分为糖皮质激素敏感（GCS）组（28例）和糖皮质激素抵抗（GCR）组（15例）；以16名健康志愿者为正常对照组。采用实时荧光定量PCR方法检测HSP70mRNA、HSP90mRNA在骨髓单个核细胞（BMMNCs）中的表达。分析患者组与正常组、GCS组和GCR组、肿瘤重度负荷组和肿瘤轻度负荷组的组间表达差异。结果（1）ALL患者组HSPT0mRNA（0．95±0．36）、HSIX）0mRNA（1．97±0．63）的表达均明显高于正常对照组（0．28±0．17，0．62±0．21，P〈0．01）；（2）GCR组的HSP70mRNA（1．09±0．22）、HSP90mRNA（2．18±0．42）的表达均明显高于GCS组（0．57±0．19，1．43±0．35，P〈0．05，P〈0．01）；（3）肿瘤重度负荷组的HSP70mRNA（0．97±0．34）、HSPg0mRNA（2．01±0．58）的表达均明显高于肿瘤轻度负荷组（0．61±0．22，1．34±0．29，P〈0．01）。结论ALL糖皮质激素抵抗患者存在HSP90及HSP70过表达；检测HSP90，HSP70的表达，对于ALL患者预后判定，化疗方案选择及疗效判定方面具有参考意义。Objectives To investigate the relationships of the expressions of heat shock protein 70 （HSP70） and HSP90 genes in bone marrow mononuclear cells （BMMNCs） with glueoeorticoid （GC）-resis- tenee （GCR） in acute lymphoblastic leukemia （ALL） patients. Methods The expressions of HSP70 and HSP90 in bone marrow mononuclear Ceils from 43 newly diagnosed ALL patients and 16 healthy volunteers were examined by real time polymerase chain reaction. Of them, 43 patients received glucocorticoid therapy were divided into GC-sensitive （GCS） group and GC-resistant（GCR） group according to GC response. Re- suits （1）The expressions of HSP70 mRNA （0. 95 ± 0. 36） and HSP90 mRNA （ 1.97 ± 0. 63 ） in BMMNCs from ALL patients were significantly higher than that in the controls [ （0. 28 ±0. 17）and （0. 62 ±0. 21 ）, P 〈0.01] ; （2）The expressions of HSP70 mRNA （1.09 ± 0. 22）and HSP90 mRNA （2. 18 ±0.42） in BMMNCs from GCR group patients were significantly higher than that in the GCS group patients [ （0. 57 ±0. 19）and （1.43 ± 0. 35 ）, P 〈 0. 05, P 〈 0. 01 ] ; （3）The expressions of HSP70 mRNA（0. 97 ± 0. 34）and HSP90 mRNA （2.01±0. 58 ） in BMMNCs from the ALL patients with high-degree tumor burden were signif- icantly higher than that in the patients with low-degree tumor burden [ （0. 61 ±0. 22） and （ 1.34 ±0. 29）, P 〈0.01 ]. Conclusions The mRNA expressions of HSP70 and HSP90 in BMMNCs from GCR group pa-tients were significantly increased. Detection of HSP90 and HSP70 expressions may help ALL patients for prognosis judgment, chemotherapy plan selection and therapeutic effect evaluation.

关 键 词：HSP70热休克蛋白质类 遗传学 HSP90热休克蛋白质类 遗传学 前体细胞淋巴母细胞白血病淋巴瘤 糖皮质激素类 

分 类 号：R733.71[医药卫生—肿瘤]