增殖性糖尿病视网膜病变患者玻璃体腺苷浓度和增殖膜中CD73表达的研究  

Vitreous level of adenosine and proliferative membrane expression of CD73 in patients with proliferative diabetic retinopathy

作　　者：郎敏[1] 袁容娣[2] 陈春林[1] 霍妍[1] 邹欢[1] 叶剑[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所眼科,重庆400042 [2]第三军医大学新桥医院眼科,重庆400037

出　　处：《第三军医大学学报》2013年第10期985-987,共3页Journal of Third Military Medical University

基　　金：第三军医大学青年创新人才基金(2010XQN37)~~

摘　　要：目的通过检测增殖性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)患者玻璃体腺苷浓度及增殖膜CD73的表达,探讨增殖性糖尿病视网膜病变与玻璃体腺苷浓度及增殖膜CD73表达之间的关系。方法收集在本院眼科行玻璃体切除手术的患者共30例,分为PDR组(共16例16眼)和对照组(孔源性视网膜脱离、黄斑前膜、特发性黄斑裂孔共14例14眼)。于患者玻璃体切除手术中收集玻璃体、视网膜增殖膜及黄斑前膜。应用高效液相荧光检测器检测患者玻璃体腺苷浓度,Western blot检测膜样本中CD73的表达。结果 PDR组玻璃体腺苷浓度及增殖膜CD73表达均高于对照组[(62.37±11.47)μmol/L vs(30.34±6.41)μmol/L,P=0.000;(0.84±0.08)vs(0.39±0.05),P=0.002];PDR组患者的年龄、糖尿病病程、术前空腹血糖均与玻璃体腺苷浓度无相关关系(r=0.255,P=0.340;r=0.318,P=0.230;r=0.341,P=0.197)。结论腺苷和CD73在PDR患者的玻璃体及增殖膜中的表达较非视网膜新生血管性疾病患者明显升高,提示二者可能参与了增殖性糖尿病视网膜病变新生血管的形成。Objective To investigate the level of adenosine in vitreum and the expression of CD73 in proliferative membrane in patients with proliferative diabetic retinopathy （PDR）. Methods Thirty patients underwent vitrectomy in the Ophthalmology at Daping Hospital were divided into two groups, including a PDR group （n=16） and a control group （n=14）. Rhegmatogenous retinal detachment, idiopathic macular hole and macular epiretinal membrane were included in the control group. The level of adenosine in vitreum was measured by HPLC, and the expression of CD73 in preretinal membranes was measured by Western blotting. Results The level of adenosine in the PDR group was significantly higher than that in the control group [（62.37±11.47）μmol/L vs （30.34±6.41）μmol/L,P=0.000]. The expression of CD73 in the PDR group was significantly higher than that in the control group （0.84±0.08 vs 0.39±0.05, P=0.002）. There was no correlation with age, course of diabetes and fasting plasma glucose in the PDR patients and the concentration of adenosine in vitreum before operation （r=0.255, P=0.340; r=0.318, P=0.230; r=0.341, P=0.197）. Conclusion Adenosine and CD73 may be involved in the progress of neovascularization in PDR.

关 键 词：腺苷 CD73 增殖性糖尿病视网膜病变 视网膜新生血管 

分 类 号：R363.21[医药卫生—病理学] R587.2[医药卫生—基础医学]