rhFSH对雄激素致不孕大鼠局灶性脑缺血大脑皮质AKT及PDGF-B基因表达的影响  

The effect of rhFSH on AKT and PDGF B gene expression in cerebral cortex of androgen induced sterility rats with focal cerebral ischemia

作　　者：周家文[1] 陆玉熊[2] 李洪岩[2] 项喜艳[2] 张勇[2] 孙连坤[2] 康劲松[2] 

机构地区：[1]吉林大学中日联谊医院妇产科,吉林长春130033 [2]吉林大学白求恩医学院病理生理学教研室,吉林长春130021

出　　处：《中国实验诊断学》2013年第5期813-816,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨重组人促卵泡激素(rhFSH)对雄激素致不孕大鼠局灶性脑缺血大脑皮质AKT及PDGF-B基因表达的影响。方法建立雄激素致不孕大鼠(ASR)模型,给予rhFSH后应用线栓法建立大鼠大脑中动脉阻塞(MCAO)模型。48只大鼠随机分为ASR+假手术(sham)组、ASR+rhFSH+sham组、ASR+M CAO组及ASR+rhFSH+MCAO组,每组12只。采用Longa法进行神经行为评分。逆转录聚合酶链反应(RT-PCR)检测脑皮质AKT及PDGF BmRNA的表达。结果 ASR+MCAO组大鼠出现明显的神经运动功能障碍体征,缺血侧大脑皮质AKT mRNA的表达降低,PDGF-B mRNA表达上调,而ASR+rhFSH+MCAO组大鼠神经运动功能障碍体征明显减轻,AKT及PDGF-B的表达均明显上调。结论 rhFSH可能通过对AKT及PDGF的调节,促进血管增生以及重塑,导致细胞存活增多,从而减轻脑损伤。Objective To investigate the effect of Recombinant Human Follicle-Stimulating Hormone （rhFSH） on AKT and PDGF B gene expression in cerebral cortex of androgen induced sterility rats with focal cerebral ischemia. Methods The androgen sterilized rats （ ASR ） model was first established,then treated with recombinant human folli- cle stimulating hormone （ rhFSH ） . Then the rats were subjected to focal middle cerebral artery occlusion （MCAO）. 48 rats were randomly divided into four groups.. ASR ^- sham group,ASR q- rhFSH q- sham group,ASR^-MCAO group and ASR＋rhFSH＋MCAO group. Neurological deficits were assessed at 0 h and 24 h after MCAO using Longa ＂s 5-point scale. The reverse transcriptase polymerase chain reaction （RT-PCR） was used to detect AKT and PDGF B mRNA expression. Results The significant neurological deficits were observed in ASR ＋ MCAO group during the ob- servation period. The AKT mRNA expression in ischemia side of ASRq-MCAO group were obviously lower than that of ASR＋sham group（P〈0.05）and the PDGF-B mRNA expression in ischemia side of ASR＋MCAO group were obvi-ously higher than that of ASR＋sham group（P〈0.05）. The neurological deficit scores in ASR ＋ rhFSH ＋ MCAO group were obviously lower than that of ASR＋MCAO group（P〈0.05）. The AKT,PDGF-B mRNA expression in is- chemia side of ASR＋rhFSH＋MCAO group were obviously higher than that of ASR--MCAO group（P〈0.05）. Conclusion These results suggested that rhFSH can lessen the brain ischemia injury by regulating of AKT and PDGF-B, then promote vascular proliferation and remodeling and increase the number of survival cortex neuron.

关 键 词：雄激素致不孕大鼠 重组人促卵泡激素 大脑中动脉阻塞AKT PDGF-B 

分 类 号：R743.3[医药卫生—神经病学与精神病学]