米糠酸化油中不皂化物、脂肪酸和磷脂的成分分析  

Analysis of unsaponifiable matter,fatty acid and phosphatide content in rice bran acid oil

作　　者：王琰[1] 邓利[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京市生物加工过程重点实验室,北京100029

出　　处：《现代化工》2013年第5期136-138,共3页Modern Chemical Industry

基　　金：国家“863”计划(2009AA03Z232);国家“973”计划(2011CB710800,2011CB200905);国家科技支撑计划(2011BAD22B04);北京市科委(Z09010300840901)

摘　　要：建立一种用气相色谱-质谱联用(GC-MS)与超临界CO2萃取结合的方法分析米糠酸化油中的不皂化物(角鲨烯、甾醇和脂肪醇)。分别以角鲨烯、甾醇、十六醇标准品为外标物,用外标标准曲线法测定角鲨烯、甾醇、脂肪醇的含量。结果表明,米糠酸化油中含有角鲨烯的质量分数为0.11%,甾醇的质量分数为1.16%,脂肪醇的质量分数为2.21%。同时,用气相色谱法对油中脂肪酸进行测定,以油酸甲酯作为参比物,十二烷作为内标物,用内标法测得脂肪酸质量分数为75.0%。油中磷脂的质量分数的测定采用灰化法和甲醇-冰乙酸萃取法,测得磷脂的质量分数为0.33%。A method for the analysis of components in supercritical CO2 extracts from rice bran acid oil by gas chromatography-mass spectrometry （GC-MS） method is developed. The contents of unsaponifiable matter in rice bran acid oil are analyzed. Squalene, sterols and fatty alcohols are determined by external standard curve method. The content of squalene,sterols and fatty alcohols in rice bran acid oil determined by this method are 0. 11% ,1.16% and 2. 21%, respectively. At the same time, the content of fatty acids in rice bran acid oil are determined by GC, with internal standard curve method, using methyl oleate as reference compound and dodecane as internal standard. The content of FFA is 75.0%. Then, the ashing method and methanol-acetic acid extraction method are applied to determine the content of phospholipids. The phosphatide in rice bran acid oil content is 0. 33%.

关 键 词：超临界CO2萃取 气相色谱-质谱 米糠酸化油 不皂化物 

分 类 号：O657.63[理学—分析化学]