血清饥饿条件下p21活化激酶6(PAK6)与雄激素受体表达的相关性及其意义  

The relationship of p21 activated kinase 6 (PAK6) and androgen receptor upon serum starvation

作　　者：刘彤[1] 李丹妮[2] 李洋[1] 李丰[1] 

机构地区：[1]中国医科大学基础医学院细胞生物教研室/教育部医学细胞生物学重点实验室,辽宁沈阳110001 [2]中国医科大学

出　　处：《解剖学研究》2013年第2期81-84,共4页Anatomy Research

基　　金：国家自然科学基金资(31171360)

摘　　要：目的血清饥饿条件诱导前列腺癌细胞系中内源性p21活化激酶6(PAK6)与雄激素受体(AR)蛋白表达,探讨PAK6与AR蛋白表达的关系。方法在前列腺癌细胞系CWR22Rv1和LNcap细胞进行血清饥饿,诱导内源性PAK6与AR蛋白表达发生变化。结果血清饥饿处理的不同时间点,CWR22Rv1和LNCaP细胞中PAK6蛋白表达呈上升趋势,AR蛋白表达呈下降趋势。瞬时转染递增剂量的PAK6引起AR蛋白表达的抑制。结论血清饥饿条件诱导前列腺癌细胞中PAK6与AR蛋白表达呈负相关。Objective To learn the expression relationship of p21-activated kinase 6 （PAK6）and androgen receptor （AR） in prostate cell line upon serum starvation. Methods Serum was abolished from medium in CWR22Rvl and LNCaP cells, and the ex- pression level of PAK6 and AR was examined. Results At the different time point of serum starvation, PAK6 was downregulated and AR was upregulated. Transient transfection with dose-increasing PAK6 induced downregulation of AR protein. Conclusion The expression of PAK6 and AR showed a negative correlation upon serum starvation in prostate cell lines.

关 键 词：P21活化激酶 雄激素受体 血清饥饿 前列腺癌细胞 

分 类 号：R737.25[医药卫生—肿瘤]