超滤分离对虾头自溶产物ACE抑制活性的影响  

Effect of ultrafiltration on ACE inhibitory activity of autolysate of shrimp head

作　　者：朱国萍[1] 章超桦[2,3] 曹文红[2,3] 吉宏武[2,3] 

机构地区：[1]广东海洋大学分析测试中心,广东湛江524088 [2]广东海洋大学食品科技学院,广东湛江524088 [3]广东省水产品加工与安全重点实验室,广东湛江524088

出　　处：《上海海洋大学学报》2013年第3期452-457,共6页Journal of Shanghai Ocean University

基　　金：国家科技支撑计划项目(2007BAD29B09);教育部科学技术重点项目(210155)

摘　　要：采用3 000 u、5 000 u、8 000 u的超滤膜对凡纳滨对虾虾头自溶产物进行超滤分级分离,研究各组超滤组分的相对分子质量分布和氨基酸组成的变化,并比较各组超滤组分的血管紧张素转换酶(angiotensin-I-converting enzyme,ACE)抑制活性。结果表明:利用超滤膜分离虾头自溶液可提高虾头自溶产物的ACE抑制活性,3 000 u超滤组分的ACE抑制活性为0.61mg/mL,活性居各超滤组分之首;8 000 u超滤膜有效载留了大部分大分子物质,相对分子质量小于3 000 u的多肽随超滤孔径的减小而增多;3 000 u超滤组分中芳香族氨基酸和支链氨基酸均高于其余各超滤组分。Litopenaeus vannamei head autolysate was consecutively extracted through ultrafiltration membrane with molecular weight cut-offs （MWCO） at 3 000, 5 000 and 8 000 u, respectively. The distribution of relative molecular mass, composition of amino acid as well as ACE （Angiotensin-I-converting enzyme ） inhibitory activity in different filtrates were measured. The results showed that ACE inhibitory activity of autolysate increased after extraction by extra-fine membrane. Obviously, the inhibitory activity of the filtrate of 3 000 u extra-fine membrane was 0. 61 mg/mL, which ranked the first compared with other filtrates. Meanwhile, the most of macromolecular substance was retained by 8 000 u while the extracted peptides with relative molecular mass less than 3 000 u increased markedly with the decline of MWCO of membrane. In addition, the contents of aromatic amino acid and branched chain amino acid in filtrate of 3 000 u were higher than those in other filtrates.

关 键 词：虾头 自溶产物 超滤 分子量分布 氨基酸 ACE抑制活性 

分 类 号：S998[农业科学—水产科学]