鱿鱼墨黑色素及黑色素铁定量分析  

Quantitative Analysis of Melanin and Melanin-Fe (Ⅲ) from Squid

作　　者：高翔[1] 李国云[1] 王静凤[1] 薛长湖[1] 王玉明[1] 李兆杰[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品科学》2013年第10期173-176,共4页Food Science

基　　金：海洋公益性行业科研专项(201105029)

摘　　要：采用高速离心法从北太平洋鱿鱼(Ommastrephes bartrami)墨囊中提取鱿鱼黑色素,并通过黑色素与FeCl3螯合制备了Fe(Ⅲ)含量为7%的黑色素铁。采用H2O2氧化降解及高效液相色谱法定量测定黑色素两种降解产物2,3-二羧酸吡咯(PDCA)和2,3,5-三羧酸吡咯(PTCA)对鱿鱼墨黑色素及黑色素铁进行定量研究。结果表明:以H2O2氧化降解黑色素产物PDCA和PTCA为基础的鱿鱼墨黑色素定量方法效果良好。利用该方法对黑色素铁进行定量仅检测出40%的黑色素,盐酸脱铁后检出量上升至75%。常规的鱿鱼墨黑色素的定量方法不适用于黑色素铁的定量分析。Melanin was extracted from the ink of North Pacific squid（Ommastrephes bartrami） by high-speed centrifugation.Squid melanin-Fe（Ⅲ） containing 7%（m/m） bound Fe（Ⅲ） was prepared by exposing squid melanin to aqueous solution of FeCl3.Melanin was degraded with H2O2 and two degradation products,pyrrole-2,3-dicarboxylic acid（PDCA） and pyrrole-2,3,5-tricarboxylic acid（PTCA） were determined by HPLC.The quantitative method to determine squid melanin based on the degradation products of melanin PTCA and PDCA exhibited desired results.Only 40% of the melanin was detected in quantitative analysis of the squid melanin-Fe（Ⅲ） by this method and increased to 75%.After hydrochloric acid treatment to expel Fe,the melanin detected rose to 75%.

关 键 词：鱿鱼墨黑色素 鱿鱼墨黑色素铁 定量分析 高效液相色谱 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]