免疫磁珠富集技术联合选择性培养基快速检测单增李斯特菌  

Rapid detection of Listeria monocytogenes by immunomagnetic separation combined with selective medium

作　　者：闻一鸣[1] 李志清[1] 童吉宇[1] 向军俭[1] 

机构地区：[1]广东省分子免疫与抗体工程重点实验室暨南大学抗体工程研究中心,广东广州510632

出　　处：《生物工程学报》2013年第5期672-680,共9页Chinese Journal of Biotechnology

基　　金：广东省部产学研结合项目(No.2010B090301037)资助~~

摘　　要：单增李斯特菌是一种危害极大的食源性致病菌，建立快速及特异的检测方法对于食品安全监控尤为重要。丈中联合免疫磁珠与选择性培养基对不同浓度（10^1～10^5CFU／mL）单增李斯特菌进行检测，并对3种李斯特菌、金黄色葡萄球菌及副溶血弧菌进行交叉试验；同时模拟食物污染，探索免疫磁珠．平板法检测样品的检测限以及该方法的最快检测时间。结果显示特异性免疫磁珠联合选择性平板法可检出浓度为10^3 CFU／mL及以上的单增李斯特菌；牛奶样品仅需6h增菌能被检出，检测限为0．7CFU／mL。联合使用免疫磁珠富集技术与选择性培养基，能在30h内完成对牛奶样品的检测，较国标法减少38h以上，且具有同等的灵敏度。Listeria monocytogenes is a pathogenic bacterium, therefore, it is essential for food safety monitoring to establish a rapid and specific detecting method. In this study, immunomagnetic beads and selective medium were combined to detect Listeria monocytogenes at different concentrations （10^1-10^5 CFU/mL）. Other three types of Listeria spp., Staphylococcus aureus and Vibrio parahaemolyticus were also detected to conduct the cross-reaction analysis. Meanwhile, contaminated milk samples were prepared to explore the limit of detection of immunomagnetic beads combining with selective medium. Results showed that Listeria monocytogenes with the concentration of 10^3 CFU/mL and above was successfully detected. Milk samples were detected within 6 hours, with a detection limit of 0.7 CFU/mL. The method developed is capable of detecting milk samples within 30 h, which is 38 h faster compared with national standard method with the same sensitivity.

关 键 词：单增李斯特菌 免疫磁珠 选择性培养基 检测限 

分 类 号：R155.5[医药卫生—营养与食品卫生学]