德氏乳杆菌WHH009高密度培养条件的研究  

Study about the high density fermentation of one lactobacillus with high quantity and fast production of the lactic acid

作　　者：李言郡[1] 葛红娟[1] 范恩宇[1] 单胜燕[1] 李宝磊[1] 侯保朝[1] 

机构地区：[1]杭州娃哈哈集团有限公司研究院生物工程研究所,杭州310018

出　　处：《中国乳品工业》2013年第5期29-32,共4页China Dairy Industry

基　　金：企业技术中心创新能力建设专项:发改委高技[2008]2012

摘　　要：通过对分离自青海地区传统发酵乳中的一株产酸快速乳杆菌Lactobacills delbrueckii subsp.lactis WHH009进行了高密度培养条件的优化。提出了适合该菌株的高密度发酵方案,认为在发酵过程中采用CO2进行搅拌,pH值维持在6.1,发酵温度采用43℃,在菌体生长对数期进行补料,最终发酵液中的活菌数可达1.5×1010mL-1,菌泥得率为2.35%,为其他乳酸菌高密度发酵研究,提供了一些参考和理论依据。The high cell density fermentation is one important step in the DVS（Direct Vat Set）production, different strains have different chatacters, and then the fermentation strategy are different. Lactobadlls delbrueckii subsp, lactis WHH009 was isolated from the traditional yoghurt of Qinghai, China. It has some advantage characters during the yoghurt fermentation, and through the study of its metalotic and fermentation characters, the final purpose was to use in the yoghurt production. This paper provide some methods and imformation on the high cell density fermentation. The best fermented condition were keeping at pH=6.1, 43 qC and feeding medium at the 3rd hour from the beginning fermentation. After 6 hours fermentation, the final cell density was 1.5×10^（10）mL^（-1） and cell slurry could be reached to 2.35%.

关 键 词：乳酸菌 产酸 高密度发酵 

分 类 号：Q93-335[生物学—微生物学]