抑癌基因p53第5～8外显子突变与喉鳞癌关系的研究  

Experimental Studies on Exons 5-8 of p53 Gene Mutation in Laryn geal Squamous Cell Carcinoma

作　　者：张晓晴[1] 刘世喜[2] 周光耀[2] 梁传余[2] 林代诚[2] 乔小明 

机构地区：[1]华西医科大学附属第四医院耳鼻咽喉科,成都610041 [2]华西医科大学附属第一医院耳鼻咽喉科

出　　处：《华西医科大学学报》2000年第3期295-297,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金(批准号39500160);美国中华医学基金会(CMB)专项基金

摘　　要：为探讨抑癌基因p53基因突变与喉鳞癌之间的关系，应用聚合酶链反应单链构象多态性分析（PCR－SSCP）银染系统，检测60例喉鳞癌新鲜组织中抑癌基因p53基因第5～8外显子的突变情况。结果：60例喉鳞癌组织中，发生47例第5～8外显子迁移率异常的SSCP区带，总的突变率为78.3%。SSCP阳性病例在外显子5、6、7、8中的突变率由高到低依次为Exon 5、Exon 7、Exon 8、Exon 6。提示第5、7外显子有可能是喉鳞癌发病相关的致癌因素选择性攻击的关键部位。This study was designed to detect the point mutations of exons 58 of p53 gene in laryngeal squamous cell carcinoma (LSCC) and analyze their relationship. The detection of fresh tumor samples from LSCC patients was performed using silver staining PCRSSCP method. From among 60 patient samples , 47 were positive in SSCP. Mutation rate was 78.3% (47/60). The results showed that the prevalence of p53 mutations in LSCC subjected to silver staining PCRSS CP test were 50% (30/60) in exon 5, 11.67%(7/60) in exon 6, 41.6%(25/60) in exo n 7, and 25%(15/60) in exon 8. The majority of the mutations were found in exon 5 and exon 7. Exon 5 and exon 7 of p53 gene may be the mutation hotspot in LSCC; t hey may be the critical position easily attacked by some carcinogen factors relati ng to LSCC.

关 键 词：喉鳞癌 P53基因 银染PCR-SSCP 基因突变 

分 类 号：R739.650.2[医药卫生—肿瘤]